A feature of much interest in the observation of cases of pernicious anemia is the behavior of the reticulated cells. In six cases of pernicious anemia, four of them treated by transfusion, which are here reported, this element of the blood-picture was studied with special care, and from these as well as from earlier cases of transfusion done in St. Luke's Hospital, certain conclusions are drawn in regard to the value of vital staining in cases of severe anemia, and concerning the subject of transfusion in general.
Ever since the discovery of the fact that by treating fresh, unfixed blood with solutions of various dyes, such as brilliant cresyl blue, polychrome methylene blue, neutral red, pyronin methylene green, etc., a curious and otherwise invisible structure could be demonstrated in the red cells, there has been much speculation as to its significance. Ehrlich, Pappenheim and Horsley were among the
VOGEL KM, McCURDY UF. BLOOD TRANSFUSION AND REGENERATION IN PERNICIOUS ANEMIA. Arch Intern Med (Chic). 1913;XII(6):707–722. doi:10.1001/archinte.1913.00070060099007