Previous studies on the distribution of adenosine triphosphatase (ATPase) have been carried out mostly on paraffin sections and have resulted largely in a critical analysis of methodology rather than in an interpretation of the results. The biochemical studies by Robin and Smith18 showed significant variation in the chemical composition of different parts of the gray areas of the central nervous system, and those of Naidoo and Pratt15 yielded different results under different conditions. Although the enzymes have been studied histochemically in brain stem,6 cerebral cortex,11,15 and spinal ganglia and cerebellum,21,22 little has been done so far on the spinal cord. The present work was undertaken on cryostat sections and using the enzyme techniques currently in favor.
Material and Method
Healthy young adult rats were killed by decapitation without anesthesia, and the pieces of spinal cord, mostly from the cervical and thoracic regions, were taken
NANDY K, BOURNE GH. Adenosine Triphosphatase and 5-Nucleotidase in Spinal CordHistochemical Study of Localization in Rat Spinal Cord. Arch Neurol. 1964;11(5):547–553. doi:10.1001/archneur.1964.00460230097009