[Skip to Content]
Access to paid content on this site is currently suspended due to excessive activity being detected from your IP address 54.87.114.118. Please contact the publisher to request reinstatement.
Sign In
Individual Sign In
Create an Account
Institutional Sign In
OpenAthens Shibboleth
[Skip to Content Landing]
Download PDF
Age distribution of children with sinovenous thrombosis (lighter area) or arterial ischemic stroke (darker area).

Age distribution of children with sinovenous thrombosis (lighter area) or arterial ischemic stroke (darker area).

Prothrombotic Testing in Children With Sinovenous Thrombosis or Arterial Ischemic Stroke*
Prothrombotic Testing in Children With Sinovenous Thrombosis or Arterial Ischemic Stroke*
1.
Butler  IJ Cerebrovascular disorders of childhood. J Child Neurol. 1993;8197- 200Article
2.
Broderick  JTalbot  TPrenger  ELeach  ABrott  T Stroke in children within a major metropolitan area: the surprising importance of intracerebral hemorrhage. J Child Neurol. 1993;8250- 255Article
3.
Schoenberg  BMellinger  JSchoenberg  D Cerebrovascular disease in infants and children: a study of incidence, clinical features, and survival. Neurology. 1978;28763- 768Article
4.
Roach  ESRiela  AR Pediatric Cerebrovascular Disorders. 2nd ed. Armonk, NY Futura Publishing Co Inc1995;
5.
Bick  RL Hypercoaguability and thrombosis. Med Clin North Am. 1994;78635- 665
6.
Wallen  PWiman  B Characterization of human plasminogen, I: on the relationship between different molecular forms of plasminogen demonstrated in plasma and found in purified preparation. Biochim Biophys Acta. 1970;22120- 30Article
7.
Andrew  MPaes  BMilner  R  et al.  Development of the human coagulation system in the full-term infant. Blood. 1987;70165- 172
8.
Andrew  MPaes  BMilner  R  et al.  Development of the human coagulation system in the healthy premature infant. Blood. 1988;721651- 1657
9.
Andrew  MVegh  PJohnston  MBowker  JOfosu  FMitchell  L Maturation of the hemostatic system during childhood. Blood. 1992;801998- 2005
10.
Dacie  SJVLewis  SM Investigation of a thrombotic tendency. Practical Haematology. 7th ed. New York, NY Churchill Livingstone Inc1991;323- 324
11.
De Stefano  VLeone  GMastrangelo  S  et al.  Clinical manifestations and management of inherited thrombophilia: retrospective analysis and follow up after diagnosis of 238 patients with congenital deficiency of antithrombin III, protein C, protein S. Thromb Haemost. 1994;72352- 358
12.
Heistinger  MRumpl  EIlliasch  H  et al.  Cerebral sinus thrombosis in a patient with hereditary protein S deficiency: case report and review of the literature. Ann Haematol. 1992;64105- 109Article
13.
Enevoldson  TPRoss Russell  RW Cerebral venous thrombosis: new causes for an old syndrome. Q J Med. 1990;771255- 1275Article
14.
Daif  AAwada  Aal Rajeh  S  et al.  Cerebral venous thrombosis in adults: a study of 40 cases from Saudi Arabia. Stroke. 1995;261193- 1195Article
15.
Matsushita  KKuriyama  YSawada  TUchida  K Cerebral infarction associated with protein C deficiency. Stroke. 1992;23108- 111Article
16.
Furlan  AJLucas  FVCraciun  RWohl  RC Stroke in a young adult with familial plasminogen disorder. Stroke. 1991;221598- 1602Article
17.
Kohler  JKasper  JWitt  Ivon Reutern  G Ischemic stroke due to protein C deficiency. Stroke. 1990;211077- 1080Article
18.
Davous  PHorellou  MHSamama  M Cerebral infarction and familial protein S deficiency. Stroke. 1990;211760- 1761Article
19.
Dahlback  B New molecular insights into the genetics of thrombophilia: resistance to activated protein C, caused by Arg506 to Gln mutation in factor V as a pathogenetic risk factor of thrombosis. Thromb Haemost. 1995;74139- 148
20.
van der Bom  JGBots  MLHaverkate  F  et al.  Reduced response to activated protein C is associated with increased risk for cerebrovascular disease. Ann Intern Med. 1996;125265- 269Article
21.
Isaacson  SNilsson  IM Defective fibrinolysis in blood and vein walls in recurrent idiopathic venous thrombosis. Acta Chir Scand. 1972;138313- 319
22.
Kennedy  CRWarner  GKai  MChisholm  M Protein C deficiency and stroke in early life. Dev Med Child Neurol. 1995;37723- 730Article
23.
Ganesan  VKelsey  HCookson  JOsborn  AKirkham  FJ Activated protein C resistance in childhood stroke. Lancet. 1996;347260Article
24.
Glueck  CJGlueck  HIGreenfield  D  et al.  Protein C and S deficiency, thrombophilia, and hypofibrinolysis: pathophysiologic causes of Legg-Perthes disease. Pediatr Res. 1994;35 ((pt 1)) 383- 388Article
25.
Rich  CGill  JCWernick  SKonkol  RJ An unusual cause of cerebral venous thrombosis in a four year old child. Stroke. 1993;24603- 605Article
26.
Broekman  AWVeltkamp  JJBertina  RM Congenital protein C deficiency and venous thromboembolism: a study of three Dutch families. N Engl J Med. 1983;309340- 344Article
27.
Brenner  BFishman  AGoldsher  DSchreibman  DTavory  S Cerebral thrombosis in a newborn with a congenital deficiency of antithrombin III. Am J Hematol. 1988;27209- 211Article
28.
Ambruso  DRJacobson  LJHathaway  WE Inherited antithrombin III deficiency and cerebral thrombosis in a child. Pediatrics. 1980;65125- 131
29.
Vomberg  PPBreederveld  CFleury  PArts  WF Cerebral thromboembolism due to antithrombin III deficiency in two children. Neuropediatrics. 1987;1842- 44Article
30.
Riikonen  RSVahtera  EMKekomäki  RM Physiological anticoagulants and activated protein C resistance in childhood stroke. Acta Paediatr. 1996;85242- 244Article
31.
Manco-Johnson  MJNuss  RKey  N  et al.  Lupus anticoagulant and protein S deficiency in children with postvaricella purpura fulminans or thrombosis. J Pediatr. 1996;128319- 323Article
32.
Levin  MEley  BSLouis  JCohen  HYoung  LHeydeman  BS Postinfectious purpura fulminans caused by an autoantibody directed against protein S. J Pediatr. 1995;127355- 363Article
33.
Minutillo  CPemberton  PJWilloughby  MLPrice  PJMarshall  LR Neonatal purpura fulminans and transient protein C deficiency. Arch Dis Child. 1990;65561- 562Article
34.
Ravelli  AMartini  ABurgio  GR Antiphospholipid antibodies in paediatrics. Eur J Pediatr. 1994;153472- 479Article
35.
The Antiphospholipid Antibodies in Stroke Group, Clinical and laboratory findings in patients with antiphospholipid antibodies and cerebral ischemia. Stroke. 1990;211268- 1273Article
36.
McNeil  HPChesterman  CNKrilis  SA Immunology and clinical importance of antiphospholipid antibodies. Adv Immunol. 1991;49193- 280
37.
Montes de Oca  MABabron  MCBletry  O  et al.  Thrombosis in systemic lupus erythematosus: a French collaborative study. Arch Dis Child. 1991;66713- 718Article
38.
Appan  SBoey  MLim  K Multiple thromboses in systemic lupus erythematosus. Arch Dis Child. 1987;62739- 741Article
39.
Roddy  SMGiang  DW Antiphospholipid antibodies and stroke in an infant. Pediatrics. 1991;87933- 935
Original Contribution
December 1998

Prothrombotic Disorders in Infants and Children With Cerebral Thromboembolism

Author Affiliations

From Hamilton Civic Hospitals Research Center (Drs deVeber, Monagle, Chan, and Andrew and Mss Lee, Vegh, and Adams) and the Department of Pediatrics, McMaster University, Hamilton, Ontario (Drs deVeber and Chan); the Divisions of Neurology (Drs deVeber, MacGregor, and Curtis) and Hematology/Oncology (Mss Adams and Marzinotto, and Drs Leaker, Massicotte, and Andrew), The Hospital for Sick Children, Toronto, Ontario; and Richardson Laboratory, Queen's University, Kingston, Ontario (Dr Lillicrap).

Arch Neurol. 1998;55(12):1539-1543. doi:10.1001/archneur.55.12.1539
Abstract

Background  To our knowledge, the contribution of prothrombotic conditions to cerebral thromboembolism has never been prospectively studied in a large series of pediatric patients.

Methods  The Hospital for Sick Children, Toronto, Ontario, established a program in January 1992 to diagnose and treat children (term newborn to 18 years old) with arterial ischemic stroke or sinovenous thrombosis. The routine evaluation for prothrombotic conditions included plasminogen, antithrombin, protein C, free protein S, activated protein C resistance, IgG and IgM anticardiolipin antibody, and lupus anticoagulant. We analyzed samples taken within 2 years of the event. We report results on patients seen from January 1, 1992, to January 1, 1997.

Results  Ninety-two patients (47 males and 45 females) entered the program during the study interval. Patients ranged from newborn to 18 years in age. Arterial ischemic stroke occurred in 78% of patients while sinovenous thrombosis occurred in 22%. All were tested for prothrombotic disorders. One or more abnormal results were present in 35 (38%) of the 92 patients. The majority (21/35) had multiple abnormal test results. The abnormal test results were anticardiolipin antibody (33%), plasminogen (9.5%), activated protein C resistance (9%), protein C (7%), antithrombin (12.5%), lupus anticoagulant (8%), and free protein S (11.5%). Male sex predicted the presence of prothrombotic abnormalities (relative risk, 1.7; 95% confidence interval, 1.2-2.5), but stroke type (relative risk, 0.8; 95% confidence interval, 0.7-1.1), age group, and presence of other risk factors did not predict abnormal testing.

Conclusions  A significant proportion (38%) of children with cerebral thromboembolism had evidence of prothrombotic conditions. In particular, there was a predominance of children with anticardiolipin antibody (33%). These data support a recommendation that children with cerebral thromboembolism be evaluated for prothrombotic disorders.

THROMBOSIS IN the central nervous system occurs in both the sinovenous system (sinovenous thrombosis) and in the arterial system (arterial ischemic stroke). The diagnosis of cerebral thrombosis in children has been facilitated by the development and availability of sensitive and safe radiographic tests such as computed tomographic scans and magnetic resonance imaging with magnetic resonance angiography.1 The widespread use of these radiographic tests in children's hospitals has contributed to an increasing awareness of thrombotic disease in the central nervous system.14

At the same time that our ability to diagnose central nervous system thrombosis has increased, our understanding of blood coagulation and both congenital and acquired defects that present risk factors for thrombotic disease has significantly improved.5 The contribution of both quantitative and qualitative abnormalities in coagulation and fibrinolysis to extracerebral thrombosis has been delineated in adult patients and is being assessed in pediatric patients.5,6 However, little is known about the association between coagulation abnormalities and cerebral thrombotic events in children. Detecting acquired and congenital prothrombotic disorders is important because this information can influence both initial and long-term therapies. In addition, family studies are indicated if a child has a congenital prethrombotic disorder. Affected patients and family members require counseling regarding risk factors for thrombosis, the need for intermittent prophylaxis in the presence of acquired risk factors, and medical care by physicians knowledgeable about their disease.

At the Hospital for Sick Children, Toronto, Ontario, specialized coagulation and stroke clinics were established in 1992 in which hematologists and neurologists collaborated in the diagnosis and management of children with thromboembolic stroke. The purpose of this article is to describe the presence of both acquired and congenital prothrombotic disorders in a consecutive cohort of pediatric patients with sinovenous thrombosis and arterial ischemic stroke seen in these specialized follow-up clinics over a 5-year period.

PATIENTS, MATERIALS, AND METHODS
PATIENT POPULATION

Consecutive children of all ages (full-term newborn to 18 years of age), with either sinovenous thrombosis or arterial ischemic stroke, admitted to the Hospital for Sick Children or referred to the follow-up Stroke Clinic at the Hospital for Sick Children between January 1, 1992, and January 1, 1997, were included in this study. No patients were excluded. All clinical, radiological, and general laboratory data were obtained as part of routine medical management. The diagnosis of sinovenous thrombosis and arterial ischemic stroke was based on clinical criteria and confirmatory computed tomographic scan, magnetic resonance imaging angiography, or venography, and/or by conventional angiography.

CLINICAL INFORMATION

Comprehensive clinical data forms were completed for each child and included the following information: age, weight, and sex, determined at the time of diagnosis; the presence of underlying or associated conditions including drug therapy; a family history of stroke or thromboembolic disease; and the type and duration of anticoagulant or antiplatelet therapy for each patient. Full clinical and radiographic details on this cohort will be published separately as part of the multicenter Canadian Pediatric Ischemic Stroke Registry analyses on arterial ischemic stroke and sinovenous thrombosis.

LABORATORY EVALUATION
General

Throughout the study period, children diagnosed as having either sinovenous thrombosis or arterial ischemic stroke were evaluated for an acquired or congenital prothrombotic condition during their in-patient stay and/or routine clinic visits, if the relevant investigations had not been performed before the clinic visit. All assay results were compared with age-appropriate normal ranges previously published by us, which used the same laboratory techniques and were based on patients from the same geographic area.79 Abnormal results from patients receiving anticoagulants that would alter test results for prothrombotic conditions were labeled indeterminate. These included protein C, protein S, and activated protein C resistance during warfarin therapy, and antithrombin during standard heparin or low-molecular-weight heparin therapy. Abnormal results of assays that may be affected by the presence of thrombus that had been taken within 1 week of a thrombotic event were also called indeterminate (proteins C and S, antithrombin, plasminogen, or activated protein C resistance). Children with indeterminate results had repeated testing performed and were reclassified as having normal or abnormal prothrombotic testing, depending on the result of the subsequent test.

Sample Processing

Venous blood (either 3 or 5 mL) was placed in a tube containing 1.05-mmol/L buffered citrate solution for a final ratio of 1 part anticoagulant to 9 parts of serum. Plasma was immediately separated from cellular elements by double centrifugation at 3000 revolutions per minute for 15 minutes at 4°C. In addition, samples to be tested for lupus anticoagulant assays were filtered through a sterile 0.020-µm screen (Acrodisk, Gelman Sciences, Ann Arbor, Mich) to achieve complete platelet removal. Plasma was subsequently aliquotted, frozen at −70°C, and assays performed in batches every 2 to 4 weeks. All laboratory results were interpreted in the context of published age-matched normal values.9

Laboratory Assays

Protein C, antithrombin, and plasminogen were measured by chromogenic assay (Coamatic Protein C Assay and Coamatic Antithrombin Assay, Ortho Diagnostic System, Raritan, NJ; Spectrolyse Plasminogen SK, Biopool, Burlington, Ontario), free protein S by enzyme-linked immunosorbent assay (Biopool Protein S EID, Biopool), and activated protein C resistance using a commercial kit (Bioclot APC, Biopool). Factor V Leiden was performed in a subset of patients by direct DNA analysis. Assays for lupus anticoagulant consisted of a dilute prothrombin time (Ortho Brain Thromboplastin, Ortho Diagnostic System), Russel Viper Venom test (Bioclot LA, Biopool), and Platelet Neutralization Procedure.10 Anticardiolipin antibody was performed by enzyme-linked immunosorbent assay of both IgG and IgM (Advanced Biological Products Inc, Mississauga, Ontario).

STATISTICAL ANALYSIS

A priori hypotheses were formulated regarding possible predictors of positive prothrombotic testing that included stroke type (arterial ischemic stroke vs sinovenous thrombosis), age at event (older infants and children aged 1 month to 18 years vs newborns [<1 month]), sex, the presence of additional underlying causes, and the presence of a positive family history for thrombotic disorders. These hypotheses were tested in bivariate analysis using 2-group comparisons. Patients with or without a prothrombotic disorder were compared using 2-tailed Fisher exact test. Results are reported as relative risk (RR), with 95% confidence intervals (CIs).

RESULTS

There were 92 children evaluated during the 5-year study period. Seventy-three had arterial ischemic stroke and 19 had sinovenous thrombosis. All patients underwent laboratory testing for 3 or more prothrombotic conditions. Thirty-five (38%) of the 92 patients had 1 or more abnormal test results for a prothrombotic condition (ie, positive prothrombotic testing) compared with 57 patients with normal test results (ie, negative prothrombotic testing).

Table 1 provides the results for the 35 patients with positive testing for a prothrombotic disorder. The presence of anticardiolipin antibody in 23 patients (33%) was the most frequent abnormality. Twelve children had anticardiolipin antibody as a single abnormality. The remainder of the children with anticardiolipin antibody also had other abnormalities, including protein S deficiency (n = 3), plasminogen deficiency (n = 2), lupus anticoagulants (n = 2), activated protein C resistance (n = 2), antithrombin deficiency (n = 1), and plasminogen and antithrombin deficiencies (n = 1). Of the remaining patients, many had combined deficiencies of coagulation proteins, including antithrombin and protein S (n = 2), antithrombin and protein C (n = 2), antithrombin and plasminogen (n = 1), antithrombin, protein C, protein S, and plasminogen (n = 2), and antithrombin, protein C, and protein S (n = 2). Two children had lupus anticoagulant and activated protein C resistance. The average interval from event to sample was 8 months (range, 1 day to 4.5 years). Of the 44 abnormal samples, 6 were taken within 1 week of the clinical event. The remainder were obtained between 7 days and 1 month (n = 10), 1 to 3 months (n = 5), 3 to 6 months (n = 4), and 6 months to 4.5 years (n = 19) after the clinical event. In patients with anticardiolipin antibody in whom samples were available from multiple time points, the results went from initially normal to abnormal in 1 patient and initially abnormal to normal in 4 patients.

Male sex was associated with positive prothrombotic testing (P=.01; RR, 1.7; 95% CI, 1.2-2.5). Of the 35 patients with positive prothrombotic testing, 25 (71%) had arterial ischemic stroke and 10 (29%) had sinovenous thrombosis (Figure 1). The distribution was not significantly different to that in the 57 patients with negative prothrombotic testing (48 [84%] with arterial ischemic stroke and 9[16%] with sinovenous thrombosis) (P = .19; RR, 0.8; 95% CI, 0.7-1.1).

The median age of children with arterial ischemic stroke was 4.9 years (age range, newborn to 15 years). The median age of the children with sinovenous thrombosis was 10 years (range, newborn to 18 years). Figure 1 shows the distribution of the entire cohort by age and stroke type. There was no significant difference in the ratio of normal to abnormal coagulation tests when analyzed by different age groups. In particular, prothrombotic abnormalities were not more likely in older infants and children (1 month to 18 years) compared with newborns (<1 month) (P = .42; RR, 1.45; 95% CI, 0.6-3.4).

The associated conditions in patients with arterial ischemic stroke and sinovenous thrombosis varied. In children with arterial ischemic stroke, cardiac disease was the most common identified underlying disease. In children with sinovenous thrombosis, dehydration was the most common precipitating factor. There was insufficient power in our study to determine whether there were any differences in underlying causes for patients with or without a prothrombotic disorder.

A positive family history for thrombotic disorders was present in only 1 of the 35 children with a prothrombotic abnormality, a child with a transient anticardiolipin antibody positivity. Four of the 57 patients with negative prothrombotic testing had family histories suggestive of a congenital prothrombotic disorder.

COMMENT

The contribution of acquired and congenital prothrombotic hemostatic disorders to thrombotic stroke in children is unknown but important to determine because of the potential risk of recurrent events, availability of specific therapies and need to screen family members. This analysis of the first 92 consecutively evaluated patients from a single institutional program showed that acquired prothrombotic hemostatic conditions exist in a large proportion of children with thrombotic stroke while congenital prothrombotic disorders are rare. Prothrombotic abnormalities were found in patients in all age groups, with both stroke types, and whether there were additional risk factors or a family history suggesting a coagulation abnormality.

The formation of a clot (thrombus) depends on a complex interaction between the coagulation and fibrinolytic systems. Congenital deficiencies of antithrombin, protein C, protein S, and the presence of activated protein C resistance predispose young adults to cerebral and noncerebral11 thrombotic events, particularly in the presence of an acquired risk factor for thrombosis. These deficiencies have all been described in adults with sinovenous thrombosis,1214 and deficiencies of plasminogen, protein C, and protein S have been reported in adults with arterial ischemic stroke.1518 Activated protein C resistance occurs in 5% of the white population,19 which increases the risk of thrombosis whether it is attributable to the factor V Leiden mutation or not.20 Deficiencies in the fibrinolytic system, for example congenital deficiency of plasminogen, also result in thrombotic complications.21

In children with stroke, deficiencies of protein C, protein S, and antithrombin,2229 and activated protein C resistance23 have been reported, although one retrospective case series30 reported no association of these abnormalities with childhood stroke. In our study, activated protein C resistance was found at only a slightly increased incidence to that reported in the general population.19 The frequency of other congenital prethrombotic conditions is difficult to determine from our study because not all patients were investigated for all abnormalities, follow-up testing was incomplete, and family studies were not performed in most cases. Further studies will be required to confirm the relative frequency of congenital prethrombotic disorders in children with stroke.

Acquired deficiencies of protein C and protein S associated with clinical thrombosis occur in patients with sepsis and viral infections such as varicella.3133 Our patients frequently had combinations of deficiencies in protein C, protein S, antithrombin, and plasminogen. The presence of multiple coagulation protein deficiencies is more consistent with an acquired than a congenital prothrombotic disorder. Because cerebral thromboses, especially arterial ischemic strokes, are usually associated with smaller thrombi than noncerebral thromboses, plasma concentrations of coagulation proteins are less likely to be significantly decreased. The multiple abnormalities in our patients were unlikely to be attributable to the sinovenous thrombosis or arterial ischemic stroke per se since the mean interval from event to testing was 8 months and abnormal hemostatic results within 1 week of the stroke event were considered to be indeterminate and subsequently repeated. Our results suggest that acquired deficiencies of coagulation proteins are present in children following thromboembolic stroke, but their causative role remains to be determined.

Antiphospholipid antibodies consist of both lupus anticoagulant and anticardiolipin antibody, and are a heterogeneous group of antibodies that react with proteins bound to phospholipids and interfere with functional coagulation tests.34 The presence of antiphospholipid antibodies is the most common acquired prothrombotic state in cerebral35 and noncerebral34,36 thrombosis in adults. Children with antiphospholipid antibodies and systemic lupus erythematosus have a significant risk of arterial or venous thrombosis, of which 50% occur in the central nervous system,37,38 and antiphospholipid antibodies have been increasingly reported in children with cerebral ischemia.39 The increased prevalence of antiphospholipid antibodies in our large cohort confirms the association between antiphospholipid antibodies and cerebrovascular disease in children. In our study antiphospholipid antibodies were found in children of all ages who did not have systemic lupus erythematosus as a primary diagnosis. Further studies are needed to confirm the importance of transient and persistent antiphospholipid antibodies and the prognostic implications of each. Whether antiphospholipid antibodies are involved in the pathogenesis of stroke remains to be proven.

In conclusion, to our knowledge, this is the first large prospective cohort study of prothrombotic abnormalities in children with arterial ischemic stroke or sinovenous thrombosis. The presence of prothrombotic abnormalities in infants and children at all ages, in both arterial ischemic stroke and sinovenous thrombosis, with and without additional risk factors or a family history suggesting a coagulation disorder suggests that all children with these forms of stroke should be evaluated for prothrombotic abnormalities. Further studies are required to define the pathogenic significance of these prothrombotic abnormalities. Finally, large prospective follow-up studies will be needed to determine if the recurrence risk of sinovenous thrombosis or arterial ischemic stroke is increased in children with prothrombotic abnormalities.

Back to top
Article Information

Accepted for publication April 3, 1998.

This work was supported by a grant from the Heart and Stroke Foundation of Ontario. Dr deVeber is supported by a stroke investigator award from the Heart and Stroke Foundation of Ontario. Dr Andrew is a career scientist with the Heart and Stroke Foundation of Canada.

We thank C. Patterson, MA, for her comprehensive data collection and C. Sparling, ART, for her technical expertise.

Reprints: Gabrielle deVeber, MD, Division of Neurology, Hospital for Sick Children, 555 University Ave, Toronto, Ontario, Canada M5G 1X8 (e-mail: deveber@sickkids.on.ca).

References
1.
Butler  IJ Cerebrovascular disorders of childhood. J Child Neurol. 1993;8197- 200Article
2.
Broderick  JTalbot  TPrenger  ELeach  ABrott  T Stroke in children within a major metropolitan area: the surprising importance of intracerebral hemorrhage. J Child Neurol. 1993;8250- 255Article
3.
Schoenberg  BMellinger  JSchoenberg  D Cerebrovascular disease in infants and children: a study of incidence, clinical features, and survival. Neurology. 1978;28763- 768Article
4.
Roach  ESRiela  AR Pediatric Cerebrovascular Disorders. 2nd ed. Armonk, NY Futura Publishing Co Inc1995;
5.
Bick  RL Hypercoaguability and thrombosis. Med Clin North Am. 1994;78635- 665
6.
Wallen  PWiman  B Characterization of human plasminogen, I: on the relationship between different molecular forms of plasminogen demonstrated in plasma and found in purified preparation. Biochim Biophys Acta. 1970;22120- 30Article
7.
Andrew  MPaes  BMilner  R  et al.  Development of the human coagulation system in the full-term infant. Blood. 1987;70165- 172
8.
Andrew  MPaes  BMilner  R  et al.  Development of the human coagulation system in the healthy premature infant. Blood. 1988;721651- 1657
9.
Andrew  MVegh  PJohnston  MBowker  JOfosu  FMitchell  L Maturation of the hemostatic system during childhood. Blood. 1992;801998- 2005
10.
Dacie  SJVLewis  SM Investigation of a thrombotic tendency. Practical Haematology. 7th ed. New York, NY Churchill Livingstone Inc1991;323- 324
11.
De Stefano  VLeone  GMastrangelo  S  et al.  Clinical manifestations and management of inherited thrombophilia: retrospective analysis and follow up after diagnosis of 238 patients with congenital deficiency of antithrombin III, protein C, protein S. Thromb Haemost. 1994;72352- 358
12.
Heistinger  MRumpl  EIlliasch  H  et al.  Cerebral sinus thrombosis in a patient with hereditary protein S deficiency: case report and review of the literature. Ann Haematol. 1992;64105- 109Article
13.
Enevoldson  TPRoss Russell  RW Cerebral venous thrombosis: new causes for an old syndrome. Q J Med. 1990;771255- 1275Article
14.
Daif  AAwada  Aal Rajeh  S  et al.  Cerebral venous thrombosis in adults: a study of 40 cases from Saudi Arabia. Stroke. 1995;261193- 1195Article
15.
Matsushita  KKuriyama  YSawada  TUchida  K Cerebral infarction associated with protein C deficiency. Stroke. 1992;23108- 111Article
16.
Furlan  AJLucas  FVCraciun  RWohl  RC Stroke in a young adult with familial plasminogen disorder. Stroke. 1991;221598- 1602Article
17.
Kohler  JKasper  JWitt  Ivon Reutern  G Ischemic stroke due to protein C deficiency. Stroke. 1990;211077- 1080Article
18.
Davous  PHorellou  MHSamama  M Cerebral infarction and familial protein S deficiency. Stroke. 1990;211760- 1761Article
19.
Dahlback  B New molecular insights into the genetics of thrombophilia: resistance to activated protein C, caused by Arg506 to Gln mutation in factor V as a pathogenetic risk factor of thrombosis. Thromb Haemost. 1995;74139- 148
20.
van der Bom  JGBots  MLHaverkate  F  et al.  Reduced response to activated protein C is associated with increased risk for cerebrovascular disease. Ann Intern Med. 1996;125265- 269Article
21.
Isaacson  SNilsson  IM Defective fibrinolysis in blood and vein walls in recurrent idiopathic venous thrombosis. Acta Chir Scand. 1972;138313- 319
22.
Kennedy  CRWarner  GKai  MChisholm  M Protein C deficiency and stroke in early life. Dev Med Child Neurol. 1995;37723- 730Article
23.
Ganesan  VKelsey  HCookson  JOsborn  AKirkham  FJ Activated protein C resistance in childhood stroke. Lancet. 1996;347260Article
24.
Glueck  CJGlueck  HIGreenfield  D  et al.  Protein C and S deficiency, thrombophilia, and hypofibrinolysis: pathophysiologic causes of Legg-Perthes disease. Pediatr Res. 1994;35 ((pt 1)) 383- 388Article
25.
Rich  CGill  JCWernick  SKonkol  RJ An unusual cause of cerebral venous thrombosis in a four year old child. Stroke. 1993;24603- 605Article
26.
Broekman  AWVeltkamp  JJBertina  RM Congenital protein C deficiency and venous thromboembolism: a study of three Dutch families. N Engl J Med. 1983;309340- 344Article
27.
Brenner  BFishman  AGoldsher  DSchreibman  DTavory  S Cerebral thrombosis in a newborn with a congenital deficiency of antithrombin III. Am J Hematol. 1988;27209- 211Article
28.
Ambruso  DRJacobson  LJHathaway  WE Inherited antithrombin III deficiency and cerebral thrombosis in a child. Pediatrics. 1980;65125- 131
29.
Vomberg  PPBreederveld  CFleury  PArts  WF Cerebral thromboembolism due to antithrombin III deficiency in two children. Neuropediatrics. 1987;1842- 44Article
30.
Riikonen  RSVahtera  EMKekomäki  RM Physiological anticoagulants and activated protein C resistance in childhood stroke. Acta Paediatr. 1996;85242- 244Article
31.
Manco-Johnson  MJNuss  RKey  N  et al.  Lupus anticoagulant and protein S deficiency in children with postvaricella purpura fulminans or thrombosis. J Pediatr. 1996;128319- 323Article
32.
Levin  MEley  BSLouis  JCohen  HYoung  LHeydeman  BS Postinfectious purpura fulminans caused by an autoantibody directed against protein S. J Pediatr. 1995;127355- 363Article
33.
Minutillo  CPemberton  PJWilloughby  MLPrice  PJMarshall  LR Neonatal purpura fulminans and transient protein C deficiency. Arch Dis Child. 1990;65561- 562Article
34.
Ravelli  AMartini  ABurgio  GR Antiphospholipid antibodies in paediatrics. Eur J Pediatr. 1994;153472- 479Article
35.
The Antiphospholipid Antibodies in Stroke Group, Clinical and laboratory findings in patients with antiphospholipid antibodies and cerebral ischemia. Stroke. 1990;211268- 1273Article
36.
McNeil  HPChesterman  CNKrilis  SA Immunology and clinical importance of antiphospholipid antibodies. Adv Immunol. 1991;49193- 280
37.
Montes de Oca  MABabron  MCBletry  O  et al.  Thrombosis in systemic lupus erythematosus: a French collaborative study. Arch Dis Child. 1991;66713- 718Article
38.
Appan  SBoey  MLim  K Multiple thromboses in systemic lupus erythematosus. Arch Dis Child. 1987;62739- 741Article
39.
Roddy  SMGiang  DW Antiphospholipid antibodies and stroke in an infant. Pediatrics. 1991;87933- 935
×