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Figure.
Signaling mechanisms involved in the neuroprotective actions of minocycline. See the text for detailed descriptions. AIF indicates apoptosis-inducing factor; BCL-2, B-cell leukemia/lymphoma 2; CCR5, chemokine receptor type 5; CXCR3, chemokine (CXC motif) receptor 3; GluR, glutamate receptor; IL, interleukin; IP-10, interferon-inducible protein 10; MAPK, mitogen-activated protein kinase; MIP-1α, macrophage inflammatory protein 1α; MMP, matrix metalloprotease; PBR, peripheral benzodiazepine receptor; TNF, tumor necrosis factor; TUNEL, terminal deoxynucleotidyl transferase dUTP (2´-deoxyuridine, 5´-triphosphate)–biotin nick-end labeling.

Signaling mechanisms involved in the neuroprotective actions of minocycline. See the text for detailed descriptions. AIF indicates apoptosis-inducing factor; BCL-2, B-cell leukemia/lymphoma 2; CCR5, chemokine receptor type 5; CXCR3, chemokine (CXC motif) receptor 3; GluR, glutamate receptor; IL, interleukin; IP-10, interferon-inducible protein 10; MAPK, mitogen-activated protein kinase; MIP-1α, macrophage inflammatory protein 1α; MMP, matrix metalloprotease; PBR, peripheral benzodiazepine receptor; TNF, tumor necrosis factor; TUNEL, terminal deoxynucleotidyl transferase dUTP (2´-deoxyuridine, 5´-triphosphate)–biotin nick-end labeling.

Table 1. 
Results of Experimental Studies on Minocycline Treatment for Neurological Diseases
Results of Experimental Studies on Minocycline Treatment for Neurological Diseases
Table 2. 
Summary of Human Trials of Minocycline Treatment for Neurological Diseases
Summary of Human Trials of Minocycline Treatment for Neurological Diseases
1.
Stirling  DPKoochesfahani  KMSteeves  JDTetzlaff  W Minocycline as a neuroprotective agent. Neuroscientist 2005;11 (4) 308- 322
PubMed
2.
Kim  HSSuh  YH Minocycline and neurodegenerative diseases. Behav Brain Res 2009;196 (2) 168- 179
PubMed
3.
Guo  GBhat  NR p38alpha MAP kinase mediates hypoxia-induced motor neuron cell death: a potential target of minocycline's neuroprotective action. Neurochem Res 2007;32 (12) 2160- 2166
PubMed
4.
Garcia-Martinez  EMSanz-Blasco  SKarachitos  A  et al.  Mitochondria and calcium flux as targets of neuroprotection caused by minocycline in cerebellar granule cells. Biochem Pharmacol 2010;79 (2) 239- 250
PubMed
5.
Antonenko  YNRokitskaya  TICooper  AJKrasnikov  BF Minocycline chelates Ca2+, binds to membranes, and depolarizes mitochondria by formation of Ca2+-dependent ion channels. J Bioenerg Biomembr 2010;42 (2) 151- 163
PubMed
6.
Matsukawa  NYasuhara  THara  K  et al.  Therapeutic targets and limits of minocycline neuroprotection in experimental ischemic stroke. BMC Neurosci 2009;10126
PubMed
7.
Alano  CCKauppinen  TMValls  AVSwanson  RA Minocycline inhibits poly(ADP-ribose) polymerase-1 at nanomolar concentrations. Proc Natl Acad Sci U S A 2006;103 (25) 9685- 9690
PubMed
8.
Guimarães  JSFreire  MALima  RRPicanço-Diniz  CWPereira  AGomes-Leal  W Minocycline treatment reduces white matter damage after excitotoxic striatal injury. Brain Res 2010;1329182- 193
PubMed
9.
Yrjänheikki  JTikka  TKeinänen  RGoldsteins  GChan  PHKoistinaho  J A tetracycline derivative, minocycline, reduces inflammation and protects against focal cerebral ischemia with a wide therapeutic window. Proc Natl Acad Sci U S A 1999;96 (23) 13496- 13500
PubMed
10.
Tsuji  MWilson  MALange  MSJohnston  MV Minocycline worsens hypoxic-ischemic brain injury in a neonatal mouse model. Exp Neurol 2004;189 (1) 58- 65
PubMed
11.
Carty  MLWixey  JAColditz  PBBuller  KM Post-insult minocycline treatment attenuates hypoxia-ischemia-induced neuroinflammation and white matter injury in the neonatal rat: a comparison of two different dose regimens. Int J Dev Neurosci 2008;26 (5) 477- 485
PubMed
12.
Lechpammer  MManning  SMSamonte  F  et al.  Minocycline treatment following hypoxic/ischaemic injury attenuates white matter injury in a rodent model of periventricular leucomalacia. Neuropathol Appl Neurobiol 2008;34 (4) 379- 393
PubMed
13.
Du  YMa  ZLin  S  et al.  Minocycline prevents nigrostriatal dopaminergic neurodegeneration in the MPTP model of Parkinson's disease. Proc Natl Acad Sci U S A 2001;98 (25) 14669- 14674
PubMed
14.
Radad  KMoldzio  RRausch  WD Minocycline protects dopaminergic neurons against long-term rotenone toxicity. Can J Neurol Sci 2010;37 (1) 81- 85
PubMed
15.
Diguet  EFernagut  POWei  X  et al.  Deleterious effects of minocycline in animal models of Parkinson's disease and Huntington's disease. Eur J Neurosci 2004;19 (12) 3266- 3276
PubMed
16.
Yang  LSugama  SChirichigno  JW  et al.  Minocycline enhances MPTP toxicity to dopaminergic neurons. J Neurosci Res 2003;74 (2) 278- 285
PubMed
17.
Peng  JXie  LStevenson  FFMelov  SDi Monte  DAAndersen  JK Nigrostriatal dopaminergic neurodegeneration in the weaver mouse is mediated via neuroinflammation and alleviated by minocycline administration. J Neurosci 2006;26 (45) 11644- 11651
PubMed
18.
Chen  MOna  VOLi  M  et al.  Minocycline inhibits caspase-1 and caspase-3 expression and delays mortality in a transgenic mouse model of Huntington disease. Nat Med 2000;6 (7) 797- 801
PubMed
19.
Smith  DLWoodman  BMahal  A  et al.  Minocycline and doxycycline are not beneficial in a model of Huntington's disease. Ann Neurol 2003;54 (2) 186- 196
PubMed
20.
Stack  ECSmith  KMRyu  H  et al.  Combination therapy using minocycline and coenzyme Q10 in R6/2 transgenic Huntington's disease mice. Biochim Biophys Acta 2006;1762 (3) 373- 380
PubMed
21.
Ryu  JKChoi  HBMcLarnon  JG Combined minocycline plus pyruvate treatment enhances effects of each agent to inhibit inflammation, oxidative damage, and neuronal loss in an excitotoxic animal model of Huntington's disease. Neuroscience 2006;141 (4) 1835- 1848
PubMed
22.
Fan  RXu  FPreviti  ML  et al.  Minocycline reduces microglial activation and improves behavioral deficits in a transgenic model of cerebral microvascular amyloid. J Neurosci 2007;27 (12) 3057- 3063
PubMed
23.
Seabrook  TJJiang  LMaier  MLemere  CA Minocycline affects microglia activation, Abeta deposition, and behavior in APP-tg mice. Glia 2006;53 (7) 776- 782
PubMed
24.
Nikodemova  MLee  JFabry  ZDuncan  ID Minocycline attenuates experimental autoimmune encephalomyelitis in rats by reducing T cell infiltration into the spinal cord. J Neuroimmunol 2010;219 (1-2) 33- 37
PubMed
25.
Zhu  SStavrovskaya  IGDrozda  M  et al.  Minocycline inhibits cytochrome c release and delays progression of amyotrophic lateral sclerosis in mice. Nature 2002;417 (6884) 74- 78
PubMed
26.
Cai  ZYYan  YChen  R Minocycline reduces astrocytic reactivation and neuroinflammation in the hippocampus of a vascular cognitive impairment rat model. Neurosci Bull 2010;26 (1) 28- 36
PubMed
27.
Liu  ZFan  YWon  SJ  et al.  Chronic treatment with minocycline preserves adult new neurons and reduces functional impairment after focal cerebral ischemia. Stroke 2007;38 (1) 146- 152
PubMed
28.
Zemke  DMajid  A The potential of minocycline for neuroprotection in human neurologic disease. Clin Neuropharmacol 2004;27 (6) 293- 298
PubMed
29.
Lampl  YBoaz  MGilad  R  et al.  Minocycline treatment in acute stroke: an open-label, evaluator-blinded study. Neurology 2007;69 (14) 1404- 1410
PubMed
30.
Casha  SZygun  DMcGowan  DYong  VWHurlbert  JR Neuroprotection with minocycline after spinal cord injury: results of a double blind, randomized, controlled pilot study. Neurosurgery 2009;65 (2) 410- 41110.1227/01.neu.0000358700.03703.a5
31.
Metz  LMLi  DTraboulsee  A  et al. GA/minocycline study investigators, Glatiramer acetate in combination with minocycline in patients with relapsing-remitting multiple sclerosis: results of a Canadian, multicenter, double-blind, placebo-controlled trial. Mult Scler 2009;15 (10) 1183- 1194
PubMed
32.
NINDS NET-PD Investigators, A pilot clinical trial of creatine and minocycline in early Parkinson disease: 18-month results. Clin Neuropharmacol 2008;31 (3) 141- 150
PubMed
33.
Bonelli  RMHeuberger  CReisecker  F Minocycline for Huntington's disease: an open label study. Neurology 2003;60 (5) 883- 884
PubMed
34.
Thomas  MAshizawa  TJankovic  J Minocycline in Huntington's disease: a pilot study. Mov Disord 2004;19 (6) 692- 695
PubMed
35.
Gordon  PHMoore  DHMiller  RG  et al. Western ALS Study Group, Efficacy of minocycline in patients with amyotrophic lateral sclerosis: a phase III randomised trial. Lancet Neurol 2007;6 (12) 1045- 1053
PubMed
36.
Zabad  RKMetz  LMTodoruk  TR  et al.  The clinical response to minocycline in multiple sclerosis is accompanied by beneficial immune changes: a pilot study. Mult Scler 2007;13 (4) 517- 526
PubMed
37.
Zhang  YMetz  LMYong  VW  et al.  Pilot study of minocycline in relapsing-remitting multiple sclerosis. Can J Neurol Sci 2008;35 (2) 185- 191
PubMed
38.
Bonelli  RMHödl  AKHofmann  PKapfhammer  HP Neuroprotection in Huntington's disease: a 2-year study on minocycline. Int Clin Psychopharmacol 2004;19 (6) 337- 342
PubMed
39.
Huntington Study Group, Minocycline safety and tolerability in Huntington disease. Neurology 2004;63 (3) 547- 549
PubMed
40.
Manev  HManev  R Interactions with GluR1 AMPA receptors could influence the therapeutic usefulness of minocycline in ALS. Amyotroph Lateral Scler 2009;10 (5-6) 416- 417
PubMed
Neurological Review
December 2010

Prospects for Minocycline Neuroprotection

Author Affiliations
 

DAVID E.PLEASUREMDAuthor Affiliations: Departments of Cell Biology and Human Anatomy (Drs Plane, Shen, and Deng), Pediatrics and Neurology (Dr Pleasure), University of California, Davis, School of Medicine; and the Institute for Pediatric Regenerative Medicine, Shriners Hospital and University of California, Davis, School of Medicine, Sacramento (Drs Deng and Pleasure).

Arch Neurol. 2010;67(12):1442-1448. doi:10.1001/archneurol.2010.191

The lack of robust treatment options available for neurodegenerative diseases, stroke, and central nervous system (CNS) trauma, all of which often involve both inflammation and apoptotic cell death, has led to an explosion of studies to reexamine the potential of established drugs for treatment of these conditions. Minocycline is a second-generation tetracycline derivative with a proven, safe clinical track record as an antibiotic and anti-inflammatory drug for treating acne and arthritis. Minocycline effectively crosses the blood-brain barrier and has demonstrated neuroprotective qualities in experimental models of CNS trauma, stroke, spinal cord injury, and neurodegenerative diseases including amyotrophic lateral sclerosis, Huntington disease, Parkinson disease, and multiple sclerosis. Thus, it is not surprising that several off-label minocycline clinical trials are under way for a variety of CNS diseases. The emerging success of some of these trials raises optimism that minocycline treatment may soon be translated into clinical practice for CNS diseases, whereas others (eg, in amyotrophic lateral sclerosis) have raised red flags. A thorough understanding of minocycline's modes of action and the targeted cellular mechanisms is warranted, as are guidelines on safe and effective doses, routes of administration, and establishment of a therapeutic window. Although several lines of evidence point to a convergent action of minocycline in suppression of both apoptosis and CNS inflammation by preventing neural cell death and by inhibiting microglial activation, the exact molecular targets of minocycline have yet to be identified and characterized. This review provides an overview of the established mechanisms of action by which minocycline exerts its neuroprotective effects, summarizes results of animal studies in experimental models of neurological diseases, and discusses results of clinical trials.

MECHANISMS OF ACTION

Several mechanisms of action are thought to be responsible for minocycline's neuroprotective effects; however, the primary target of this drug in the nervous system remains unknown. Recent studies have focused on the anti-inflammatory, antiapoptotic, and antioxidant properties of minocycline. The main biological effects of minocycline, which are involved in the pathogenesis of several neurological disorders, include inhibition of microglial activation, attenuation of apoptosis, and suppression of reactive oxygen species production.

In experimental disease models, minocycline treatment suppresses microglial proliferation/activation as well as subsequent release of cytokines such as interleukins 1β and 6 and tumor necrosis factor α.1,2 In addition, it decreases CNS expression of chemokines and their receptors such as macrophage inflammatory protein 1α, interferon-inducible protein 10, chemokine receptor type 5, and chemokine (CXC motif) receptor 3 as well as suppressing the release of lipids and the activity of matrix metalloproteases, which disrupt the blood-brain barrier.1,2 Furthermore, minocycline inhibits T-cell migration into the CNS. The neuroprotection afforded by minocycline treatment of experimental disease models also appears to be related to p38 mitogen-activated protein kinase inhibition, which both preserves neurons and inhibits microglial activation.1,3

The antiapoptotic effects of minocycline are exerted, at least in part, on mitochondria. By reducing mitochondrial calcium overloading, minocycline stabilizes the mitochondrial membrane and inhibits release of cytochrome c and other apoptotic factors into the cytoplasm, thus resulting in decreased caspase activation and nuclear damage.4,5 Minocycline also exerts caspase-independent neuroprotective effects including upregulation of antiapoptotic factor BCL-2.6

Minocycline possesses antioxidant properties in addition to its antibiotic, antiapoptotic, and anti-inflammatory properties. It directly scavenges free radicals and inhibits molecules such as cyclooxygenase 2, induced nitric oxide synthase, and nicotinamide adenine dinucleotide phosphate oxidase. The antioxidant activity of minocycline may be derived from its chemical structure or its modulating effects on enzymes such as nitric oxide synthase and lipoxygenases.

Poly (ADP [adenosine diphosphate]–ribose) polymerase-1 (PARP-1) is activated by DNA damage and is critically involved in excitotoxicity, oxidative stress, and inflammation. Activation of PARP-1 leads to translocation of apoptosis-inducing factor from the mitochondria to the nucleus and finally promotes cell death and inflammation. Alano et al7 reported that minocycline and other tetracyclines inhibited PARP-1 at nanomolar concentrations, and minocycline protected neurons against PARP-1–mediated toxicity. Other minocycline effects include inhibition of mitochondrial peripheral benzodiazepine receptor (recently renamed 18 kDa translocator protein), increased phosphorylation, membranal insertion of glutamate receptor 1, and inhibition of mitogen activated protein kinases.

Minocycline treatment provides neuroprotection against excitotoxic insults in a number of experimental models. The neuroprotective effects of minocycline are evident after glutamate exposure, and oligodendrocyte survival is enhanced by minocycline treatment after lipopolysaccharide-induced inflammation; all likely occur as a result of decreased microglial activation.8 Overall, although it is still unclear which of these mechanisms plays the pivotal role in eliciting the neuroprotective properties of minocycline, the major targets for minocycline neuroprotection could lie within the complex signaling network linking mitochondria, oxidative stress, excitotoxicity, PARP-1, and apoptosis (Figure).

STUDIES IN EXPERIMENTAL MODELS OF HUMAN NEUROLOGICAL DISEASES
Ischemic Injury

Yrjanheikki et al9 first published the neuroprotective effects of minocycline on cerebral ischemia, prompting numerous subsequent evaluations of minocycline on experimental models of stroke, neurodegenerative diseases, and spinal cord injury (Table 1). Some of these studies indicated substantial neuroprotective effects, while in others, minocycline was ineffective or even deleterious. These studies varied widely in dosage, route of administration, timing of drug treatment, species, and experimental model used. Several studies that tested multiple doses of minocycline treatment after ischemia reported neuroprotection with relatively low doses but observed toxicity with high-dose minocycline treatment.6,10 Interestingly, minocycline treatment exacerbated injury in neonatal mouse models of stroke, whereas it attenuated injury in rats using a similar model and dosing paradigm.10,11 Furthermore, minocycline exerts different effects on different types of cells. One recent study found that, while low-dose minocycline was protective to neurons in both in vivo and in vitro experimental models of stroke, it provided no protection to astrocytes in the same models.6 Yet, other studies describe decreased ischemia-induced astrogliosis and reduced white matter damage after ischemic injury following minocycline treatment.12,26 In addition, though most of these publications addressed only the acute effects of minocycline treatment on ischemic injury, it will be important, from a translational point of view, to determine whether minocycline treatment confers long-term neuroprotection or simply delays the onset of cell death. One promising observation, in this respect, is that ischemia-induced behavioral deficits lessened after minocycline treatment.27

Parkinson Disease

The effects of minocycline treatment on neurodegeneration in chemically induced Parkinson disease models have varied. While most studies found reduced microglial activation and neuronal cell death,13,14 others reported decreased microglial activation and enhanced neuron toxicity.15,16 In one genetic model, the Weaver mouse, minocycline treatment resulted in nigrostriatal neuroprotection.17

Huntington Disease

In a genetic model of Huntington disease (HD), the R6/2 mouse, one group reported that minocycline provided neuroprotection,15 another observed no clinical evidences of therapeutic efficacy,18 and a third reported that minocycline treatment was detrimental.19 Several studies investigated the effects of minocycline treatment combined with other therapies. Treatment with minocycline and pyruvate or coenzyme Q10 afforded significant neuroprotection in mice with HD,20,21 suggesting that minocycline may best be used as a combinatorial therapy.

Alzheimer Disease

While minocycline treatment produced mixed results in HD and Parkinson disease studies, it appears to be beneficial in experimental models of Alzheimer disease. Both in vivo and in vitro studies report suppressed microglial activation in Alzheimer disease models along with attenuated neuronal cell death, astrogliosis, and improved behavioral performance.22,23 Further studies are necessary to confirm that these beneficial effects lead to long-term neuroprotection.

Amyotrophic Lateral Sclerosis

Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by motor neuron death in the spinal cord, brainstem, and motor cortex. Mutations in superoxide dismutase 1 account for a proportion of cases of familial ALS, and mutant superoxide dismutase 1 transgenic mice provide a useful tool for treatment-testing paradigms. Minocycline treatment improved the symptoms of ALS in these mice via inhibition of cytochrome c release and caspase activation, thus delaying the onset of degeneration and muscle strength decline.25 Owing to the success of minocycline in these experimental trials, it quickly advanced to clinical trials but these yielded unexpectedly poor results (see “Clinical Studies” section).

Experimental Autoimmune Encephalomyelitis

Minocycline suppresses T-cell proliferation, migration, and cytokine production in an autoimmune animal model of multiple sclerosis, experimental autoimmune encephalomyelitis, and delays the onset and diminishes the severity of clinical neurological disability.24,28

Spinal Cord Injury

In animal models of spinal cord injury, minocycline treatment reduced microglial activation and astrogliosis and enhanced oligodendrocyte survival but reports of functional recovery were conflicting.2

CLINICAL STUDIES

The use of minocycline as an antibiotic as well as its excellent safety profile and penetration into the CNS spurred interest in using this drug to treat neurological diseases. Clinical trials are under way for the use of minocycline to treat several neurological diseases such as stroke, multiple sclerosis, spinal cord injury, Huntington disease, and Parkinson disease. Trials already completed have revealed both positive and negative effects (Table 2).

Brain Ischemia

A number of completed clinical studies reported beneficial effects of minocycline treatment without increased incidence of severe adverse events. In an open-label, evaluator-blinded study, oral administration of 200 mg/d of minocycline for 5 days 6 to 24 hours after acute CNS infarction caused no major complications, significantly decreased scores on the National Institutes of Health Stroke Scale and modified Rankin Scale, and increased Barthel Index scores in treated patients at days 7, 30, and 90 of follow-up. These observations suggest that the treatment of acute stroke with minocycline at this stage is associated with better clinical outcome.29 There are, however, several limitations to this study including the open-label design and small sample size. Future studies of minocycline administration after CNS infarction should include double-blind, controlled trials that use a broader window of treatment time (especially earlier) as well as oral and intravenous dosages.

Spinal Cord Injury

A small, phase 1/2 pilot study is under way to assess minocycline treatment in human patients after spinal cord injury. In this randomized, double-blind trial, patients receive 200 or 400 mg of minocycline intravenously twice daily for 7 days and are evaluated for 1 year. Results obtained thus far indicate that minocycline treatment enhances recovery, as shown by improved scores on the American Spinal Injury Association examination, Functional Independence Measure, Spinal Cord Independence Measure, London Handicap Score, and Short Form–36. These improvements were limited to patients with cervical spinal cord injury, whereas those with thoracic complete spinal cord injury showed no treatment effect.30 The sample size in this study is small, so conclusions of neuroprotection are preliminary.

Multiple Sclerosis

Several small clinical trials found minocycline treatment to be effective in reducing lesion size and occurrence rate in relapsing-remitting multiple sclerosis.36,37 More recently, a double-blind, placebo-controlled study was completed to evaluate the effects of minocycline combined with glatiramer acetate treatment in patients with relapsing-remitting multiple sclerosis. In this study, patients received 100 mg of minocycline twice daily combined with glatiramer acetate or glatiramer acetate/placebo for 9 months. The combination of drugs reduced the total number of gadolinium-enhancing lesions, the total number of new and enlarging lesions, and the total disease burden compared with glatiramer acetate/placebo treatment.31 Although the number of patients in this study was relatively small, it provides support for the use of minocycline as a combinatorial therapy for multiple sclerosis.

Parkinson Disease

In pilot clinical trials using minocycline treatment for Parkinson disease, 200 mg/d of minocycline was given for 18 months and patients were assessed for changes in the total Unified Parkinson Disease Rating Scale and a number of other measures to identify disability and symptoms.32 The patients treated with minocycline showed neither a decline in symptoms nor any increase in adverse events.

Huntington Disease

An initial open-label study found no obvious toxicity and observed improvements in motor function in minocycline-treated patients with HD based on their Unified HD Rating Scale and Mini-Mental State Examination after 6 months of treatment.33 In a 24-month follow-up, these authors reported stabilization of motor function and amelioration of psychiatric symptoms.38 The study included 14 patients, with only 11 included in the final analyses, making it difficult to draw firm conclusions. An 8-week, double-blind, randomized, placebo-controlled study by the Huntington Study Group in which patients with HD were treated with the same dose (100 or 200 mg/d) as the Bonelli group indicated that, although minocycline was well tolerated, it had no effect on Unified HD Rating Scale scores and actually worsened scores on the Stroop Interference test.39 Another small pilot study reported similar findings of good tolerability but lack of motor improvement on the Unified HD Rating Scale after 6 months of minocycline treatment.34

Amyotrophic Lateral Sclerosis

In a completed phase III clinical trial, patients with ALS received minocycline at up to 400 mg/d for 9 months; they were evaluated on the revised ALS functional rating scale and for forced vital capacity, manual muscle testing, quality of life, survival, and drug safety. Patients treated with minocycline showed faster deterioration than the placebo group according to the ALS functional rating scale and trended toward a faster decline in forced vital capacity and manual muscle testing scores. There was also a nonsignificant increase in mortality in the minocycline treatment group as well as gastrointestinal and neurological adverse events. Despite the negative effect of minocycline treatment on disease progression and adverse events, minocycline-treated patients described similar quality-of-life scores to the placebo patients.35 The authors of this study concluded that minocycline is not a viable treatment for ALS and warned against its use to treat other neurodegenerative diseases. Among possible reasons for this disappointing result are (1) that an inadequately low dosage schedule was used and (2) that the dosage of minocycline, by increasing glutamate receptor 1 phosphorylation and membrane insertion, promoted glutamate toxicity and motor neuron loss.40

SUMMARY OF AVAILABLE CLINICAL TRIALS DATA

While the clinical trials mentioned reveal both beneficial and deleterious results of minocycline treatment, all hope is not lost regarding its use as a future therapeutic. More studies are needed to confirm the appropriate timing and dosage, which may vary by disease. Some researchers speculate that doses of 100 to 200 mg/d, typically used in current clinical trials, may be ideal for humans to alleviate symptoms without toxic consequences. Others believe that this dose is too low, given that the typical dose for animal studies ranges from 10 to 100 mg/kg/d, which would theoretically equal approximately 3 to 7 g/d for humans. The dose of 200 mg/d provided improvement for acute stroke, yet had no effect on Parkinson disease or patients with HD. Thus, the possibility remains that increasing the dose of minocycline would improve its efficacy. In addition, minocycline treatment could be combined with other drug treatments in hopes of enhancing the beneficial effects without increasing the risk of harmful adverse effects seen with high doses of minocycline treatment. This therapeutic strategy has already proved useful in preliminary studies of multiple sclerosis and may be advantageous in a number of other diseases as well.

CONCLUSIONS

The practical utility of the neuroprotective effects of minocycline, though based on a substantial body of promising in vitro and animal studies, continues to be a matter of intense debate, with contradictory evidence ranging from neuroprotection to the exacerbation of toxicity in various experimental models and human trials. Studies to date have clearly established that minocycline exerts neuroprotective effects but have also shown that the actions of this drug are complex, and that its administration can, under certain circumstances, also have neurotoxic consequences. More studies, both clinical and preclinical, and both combinatorial and sequential strategies, should be carried out to ascertain the therapeutic window and the indications for which minocycline could be useful, effective, and safe.

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Article Information

Correspondence: Wenbin Deng, PhD, Department of Cell Biology and Human Anatomy, University of California, Davis, School of Medicine, 2425 Stockton Blvd, Sacramento, CA 95817 (wbdeng@ucdavis.edu).

Accepted for Publication: June 15, 2010.

Published Online: August 9, 2010. 10.1001/archneurol.2010.191

Author Contributions: Drs Plane and Shen had full access to all of the materials in the study and take responsibility for the integrity of the data and the accuracy of the data analysis. Study concept and design: Plane, Shen, Pleasure, and Deng. Acquisition of data: Plane, Shen, Pleasure, and Deng. Analysis and interpretation of data: Plane, Shen, Pleasure, and Deng. Drafting of the manuscript: Plane, Shen, Pleasure, and Deng. Critical revision of the manuscript for important intellectual content: Plane, Shen, Pleasure, and Deng. Statistical analysis: Pleasure. Obtained funding: Plane, Shen, Pleasure, and Deng. Administrative, technical, and material support: Plane, Shen, Pleasure, and Deng. Study supervision: Pleasure and Deng.

Financial Disclosure: None reported.

Funding/Support: This study was supported in part by grants from the National Multiple Sclerosis Society, Roche Foundation for Anemia Research, and Feldstein Medical Foundation (Dr Deng); the Shriners Hospitals for Children (Drs Deng and Pleasure); and by grants R01 NS059043, R01 ES015988 (Dr Deng), and R01 NS025044 (Dr Pleasure) from the National Institutes of Health.

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Antonenko  YNRokitskaya  TICooper  AJKrasnikov  BF Minocycline chelates Ca2+, binds to membranes, and depolarizes mitochondria by formation of Ca2+-dependent ion channels. J Bioenerg Biomembr 2010;42 (2) 151- 163
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Guimarães  JSFreire  MALima  RRPicanço-Diniz  CWPereira  AGomes-Leal  W Minocycline treatment reduces white matter damage after excitotoxic striatal injury. Brain Res 2010;1329182- 193
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Tsuji  MWilson  MALange  MSJohnston  MV Minocycline worsens hypoxic-ischemic brain injury in a neonatal mouse model. Exp Neurol 2004;189 (1) 58- 65
PubMed
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Carty  MLWixey  JAColditz  PBBuller  KM Post-insult minocycline treatment attenuates hypoxia-ischemia-induced neuroinflammation and white matter injury in the neonatal rat: a comparison of two different dose regimens. Int J Dev Neurosci 2008;26 (5) 477- 485
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