To pursue the method for which my plea is made does not necessitate a thorough training in bacteriological technique. A little practice in staining cover-glass preparations both in the usual way and by Gram's method, together with a study of the pictures presented by the gonococcus and the pneumococcus, will enable one, as a rule, to make an immediate diagnosis. When we fail to find the latter in cases at all suspicious, it may be advisable to resort to cultures, but these instances will be in the great minority. To grow the gonococcus will require the aid of a trained bacteriologist, as it is probably the most difficult of all organisms to cultivate on artificial media, but fortunately the appearance of a cover-glass preparation is so characteristic that a mistake is scarcely possible to one having any training in bacterioscopy.
A look at the past . . .. Arch Ophthalmol. 1999;117(2):258. doi:10.1001/archopht.117.2.258