To the Editor We read the article titled “Efficacy and Safety of Antifungal Additives in Optisol-GS Corneal Storage Medium” by Layer et al1 with interest. In their article, the authors investigated the efficacy and safety of voriconazole and amphotericin B in reducing Candida species contamination of Optisol-GS under normal storage conditions. Layer and colleagues used 0.4% trypan blue as a vital stain to evaluate corneal endothelium toxic effects.
Goktas S, Sakarya Y, Sakarya R. Trypan Blue Vital Dye Staining vs TUNEL Technique to Detect Corneal Endothelium Toxic Effects. JAMA Ophthalmol. 2014;132(12):1491. doi:10.1001/jamaophthalmol.2014.3360