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Comment & Response
December 2014

Trypan Blue Vital Dye Staining vs TUNEL Technique to Detect Corneal Endothelium Toxic Effects

Author Affiliations
  • 1Department of Ophthalmology, Konya Training and Research Hospital, Konya, Turkey
JAMA Ophthalmol. 2014;132(12):1491. doi:10.1001/jamaophthalmol.2014.3360

To the Editor We read the article titled “Efficacy and Safety of Antifungal Additives in Optisol-GS Corneal Storage Medium” by Layer et al1 with interest. In their article, the authors investigated the efficacy and safety of voriconazole and amphotericin B in reducing Candida species contamination of Optisol-GS under normal storage conditions. Layer and colleagues used 0.4% trypan blue as a vital stain to evaluate corneal endothelium toxic effects.

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