Copyright 2014 American Medical Association. All Rights Reserved. Applicable FARS/DFARS Restrictions Apply to Government Use.
In Reply There is no gold standard or widely accepted staining protocol for assessing endothelial cell death in human corneas after storage. We followed the vital dye staining protocol proposed by Park et al,1 who validated the ability of trypan blue to detect endothelial damage by comparing corneas exposed to hydrogen peroxide with those exposed to balanced salt solution and found that the optimal dye concentration was 0.4%. The only other study, to our knowledge, of an antifungal additive in Optisol-GS also used the trypan blue technique to evaluate corneal endothelial toxic effects.2
Pruzan NL, Keenan JD, Jeng BH. Trypan Blue Vital Dye Staining vs TUNEL Technique to Detect Corneal Endothelium Toxic Effects—Reply. JAMA Ophthalmol. 2014;132(12):1491. doi:10.1001/jamaophthalmol.2014.3614