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Comment & Response
December 2014

Trypan Blue Vital Dye Staining vs TUNEL Technique to Detect Corneal Endothelium Toxic Effects—Reply

Author Affiliations
  • 1Department of Ophthalmology, University of California, San Francisco
  • 2Francis I. Proctor Foundation, University of California, San Francisco
  • 3Department of Ophthalmology, San Francisco General Hospital, San Francisco, California
  • 4Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore
JAMA Ophthalmol. 2014;132(12):1491. doi:10.1001/jamaophthalmol.2014.3614

In Reply There is no gold standard or widely accepted staining protocol for assessing endothelial cell death in human corneas after storage. We followed the vital dye staining protocol proposed by Park et al,1 who validated the ability of trypan blue to detect endothelial damage by comparing corneas exposed to hydrogen peroxide with those exposed to balanced salt solution and found that the optimal dye concentration was 0.4%. The only other study, to our knowledge, of an antifungal additive in Optisol-GS also used the trypan blue technique to evaluate corneal endothelial toxic effects.2

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