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In Reply We appreciate the clarification by Balne and colleagues. Their article did indeed appear in November 2013.1 Perhaps we should have emphasized that before their article had been published, we had presented the content of our later-published work as a peer-reviewed poster at the 2012 Annual Meeting of the American Academy of Ophthalmology in Chicago, Illinois, on November 12, 2012.2 Of note, Balne and colleagues compared their LAMP assay with multiplex PCR results published by our group.3 Second, they raised the issue of our choosing IS6110 as a target leading to the LAMP assay missing it in 3 samples. While a higher sensitivity of LAMP assay using MPB64 can only be shown in a large prospective study, to our knowledge, IS6110 is the most commonly used target owing to its multiple copy numbers in the Mycobacterium genome.
Sharma K, Sharma A, Gupta A. Loop-Mediated Isothermal Amplification for Rapid Diagnosis of Tubercular Uveitis—Reply. JAMA Ophthalmol. 2015;133(2):226. doi:10.1001/jamaophthalmol.2014.4261