April 2004

Use of Standard Hematoxylin-eosin to Stain Gouty Tophus Specimens

Author Affiliations

Copyright 2004 American Medical Association. All RightsReserved. Applicable FARS/DFARS Restrictions Apply to Government Use.2004

Arch Ophthalmol. 2004;122(4):665. doi:10.1001/archopht.122.4.665

The nice clinicopathologic case report of a gouty tophus at the lateralcanthus by Morris and Fleming1 illustratesthe difficulty of identifying urate crystals in histologic sections when tissueis fixed in formalin solution and processed routinely. The authors recommendthat if a gouty tophus is suspected, the specimen should be fixed in absolutealcohol, and the tissue should be processed and stained with an alcoholiceosin without hematoxylin. I would like to share my experience in studyingthe histologic features of a periocular gouty tophus after it was fixed inCarnoy solution and then routinely processed, embedded in paraffin, and stainedwith standard hematoxylin-eosin.

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