WHEN it was found that the use of BAL (2,3-dimercaptopropanol) was of considerable value, both prophylactically and therapeutically, in the treatment of eyes exposed to lewisite (2-chlorobetachlororinylarsine) burns,1 the problem arose as to what extent BAL penetrated through the normal or the abnormal cornea and into the anterior chamber. Since BAL essentially neutralizes the toxic heavy metal arsenic in lewisite burns, the possibility that BAL might be of importance in treatment of intoxications due to other metals, such as lead, mercury and cadmium, also warranted this study.
An accurate, yet practical, method for the quantitative estimation of BAL was investigated. The cobalt nitrate test of Kensler and Rhoads,2 the porphyrindin test of Greenstein3 and the iodometric titration of sulfhydryl groups, as used by Woodward and Fry for glutathione, were tried. The iodometric titration was chosen and used exactly as recommended by Woodward and Fry,4 except that
LEOPOLD IH, STEELE WH. PENETRATION OF LOCALLY APPLIED BAL INTO THE ANTERIOR CHAMBER OF THE RABBIT EYE. Arch Ophthalmol. 1947;38(2):192-198. doi:10.1001/archopht.1947.00900010199006