THE CULTIVATION of the viruses of inclusion conjunctivitis and trachoma in tissue culture, even of susceptible human conjunctival epithelium, has failed up to the present time. Unlike most other viruses, neither the virus of trachoma nor that of inclusion conjunctivitis has been cultivated with certainty on the developing chick embryo. The present study was undertaken in an attempt to transplant conjunctival and corneal tissue of the rabbit to the chorioallantoic membrane of the developing chick embryo and to determine whether or not the transplanted conjunctiva and cornea would support the growth of various viruses. It was hoped that the results might provide a key to the successful cultivation of the viruses of trachoma and inclusion conjunctivitis.
In 1912 Murphy1 demonstrated the possibility of grafting malignant tumors on the chorioallantois of the chick embryo. The grafts grew until the eighteenth or nineteenth day of incubation. In 1938 Goodpasture and co-workers
SEZER N. CULTIVATION OF CONJUNCTIVAL AND CORNEAL TISSUE ON THE CHORIOALLANTOIC MEMBRANE. AMA Arch Ophthalmol. 1950;44(5):703-709. doi:10.1001/archopht.1950.00910020715008