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Article
January 1951

NITROGEN FRACTIONS AS CRITERION OF PRESERVATION OF CORNEAL TISSUE

Author Affiliations

NEW YORK
Dr. Buschke died Dec. 21, 1950.; From the Ayer Foundation Ophthalmic Research Laboratory, Manhattan Eye, Ear and Throat Hospital.

AMA Arch Ophthalmol. 1951;45(1):27-31. doi:10.1001/archopht.1951.01700010030003
Abstract

QUANTITATIVE data on nonprotein nitrogen fractions in the tissue may be expected to furnish useful criteria for the state of preservation of corneal donor tissue stored under various conditions. The present report deals with data on total nonprotein nitrogen, ammonia nitrogen and amino nitrogen.

MATERIALS AND METHODS  The data were obtained from freshly excised corneas with and without incubation of the excised corneas in a moist chamber at 40 C. for six hours, and from corneas stored for three days in the whole eye in a moist chamber at 4 C. with and without subsequent incubation of the excised cornea in a moist chamber at 40 C. for six hours. Storage of the whole eye at 4 C. in a moist chamber is the most widely used method of storage of human corneas for clinical purposes. As previously reported,1 the cornea becomes progressively hydrated under these conditions. It was of

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