IN ORDER that a sterile, moist donor cornea may be assured in corneal transplantations, a two-section plastic moist chamber with a top has been devised.1
The base of the moist chamber (Fig., A) is lined with sterile isotonic sodium chloride-soaked gauze and is deep enough to hold the donor eye.
A graft of suitable size and thickness is excised from the cornea after first outlining the disk with a trephine. In lamellar keratoplasty, a special knife2 is inserted to the desired corneal depth and, with a gentle, firm sawing motion, the graft is freed to the upper extremity of the outlined disk, then to the lower edge. Before the graft is completely excised, two double-armed nonabsorbable surgical (silk) sutures are inserted through the anterior edge of the corneal disk at the opposite extremities of the horizontal meridian. The graft may also be dissected with the Paufique and Desmarres
BERENS C. CORNEAL TRANSPLANTATIONSpecial Reference to Plastic Moist Chamber for Donor Eye. AMA Arch Ophthalmol. 1952;48(5):634-635. doi:10.1001/archopht.1952.00920010645013