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Article
July 1954

APPARATUS FOR CHEMICAL EXTRACTION OF SMALL PIECES OF CORNEA

Author Affiliations

BOSTON
From the Howe Laboratory of Ophthalmology, Harvard Medical School.

AMA Arch Ophthalmol. 1954;52(1):131. doi:10.1001/archopht.1954.00920050133018

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Abstract

THE SIMPLE apparatus shown in the Figure has been found eminently satisfactory for exhaustive extraction of cornea with aqueous solutions. It was developed after we had been unable to find a suitable extractor described in the literature. It has been employed in determining cations in corneal stroma after exposure to various alkalis. The extractor should prove useful in assaying dissolved or bound ions or uncharged molecules in any small sample of firm, permeable material which is not disintegrated by the extracting medium. Rapid and near-quantitative removal of individual portions of solvent is possible with a minimum of manipulation.

A 1 mm. bore capillary is sealed to the end of a 70 mm. length of 14 mm. I. D. Pyrex tubing and bent in the shape shown in the Figure. A "dimple" may be made just above the capillary to prevent plugging.

In our particular application, the exposed tissue (0.1 to

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