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Article
November 1954

EFFECT OF ACETAZOLEAMIDE (DIAMOX) ON CARBONIC ANHYDRASE ACTIVITY OF ANTERIOR UVEA OF THE RABBIT EYE

Author Affiliations

With the Technical Assistance of John L. Sawyer PHILADELPHIA
From the Research Department, the Wills Eye Hospital.

AMA Arch Ophthalmol. 1954;52(5):758-762. doi:10.1001/archopht.1954.00920050764011
Abstract

THE DRAMATIC effect of acetazoleamide (Diamox; 2-acetylamino-1,3,4thiadiazole-5-sulfonamide), a potent carbonic anhydrase inhibitor, in lowering intraocular pressure * has focused attention upon the possible role of the enzyme in the secretory mechanism of the ciliary body and iris. This association is particularly suggestive in view of the report by Wistrand2 of the presence of carbonic anhydrase activity in the ciliary body and iris of the rabbit eye. It was deemed of fundamental importance, therefore, to determine whether the administration of acetazoleamide affected the carbonic anhydrase activity of the ciliary body and iris.

METHODS  Carbonic anhydrase activity was measured in a Warburg apparatus at 1 to 2 C. according to the method of Krebs and Roughton.3 The amount of blood in the tissue was determined with the benzidine reaction, according to Ashby and Chan.4 Known dilutions of venous blood, drawn from the same rabbit from which the tissue in question

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