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Article
June 1956

Filter-Paper Electrophoresis of TearsII. Animal Tears and the Presence of a "Slow-Moving Lysozyme"

Author Affiliations

San Francisco
Department of Surgery (Ophthalmology), Stanford University Medical School (Dr. Erickson). Francis I. Proctor Foundation for Research in Ophthalmology, University of California Medical Center (Miss Feeney and Dr. McEwen).

AMA Arch Ophthalmol. 1956;55(6):800-806. doi:10.1001/archopht.1956.00930030804005
Abstract

Goldsworthy and Florey1 studied the lysozyme content of the tears of several species of animals. They used the reduction in turbidity of a suspension of Micrococcus lysodeikticus as a measure of the amount of lysozyme. They showed the absence of lysozyme in the tears of the cat and the rabbit. The tears of the goat and the dog had a small amount of lysozyme, which was about 100 to 1000 times less than the amount in normal human tears. It has been shown previously2 that the tear fluid from a single subject may be analyzed by filter-paper electrophoresis. This method shows both the lysozyme component, which appears as a distinctive protein moving toward the cathode, and the other protein components of tears. Although there has been no report on the different protein components of tears of animals, Geinitz3 studied the proteins of the blood serum of animals

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