It was the purpose of this study to investigate the regeneration of the corneal endothelium under normal conditions and during wound healing.
A. Regeneration Under Normal Conditions
Cells which line the outer or inner surfaces of the body undergo gradual degenerative changes and are finally desquamated from the surface, while new cells are formed by mitosis or amitosis. Little is known about this process in the endothelium of the cornea.
—Flat mounts of the corneal endothelium of 10 normal rabbit eyes were obtained by peeling off a very thin layer from the posterior corneal surface, including endothelium and Descemet's membrane. These preparations were about 50μ thick and were stained with hematoxylin only. The area of the posterior corneal surface thus obtained measured about 80 sq. mm. A grid in the eyepiece of the microscope was used for counting the endothelial cells under high magnification.
BINDER RF, BINDER HF. Regenerative Processes in the Endothelium of the Cornea. AMA Arch Ophthalmol. 1957;57(1):11-13. doi:10.1001/archopht.1957.00930050013003