The study of lens epithelium in tissue culture was started by Daniel B. Kirby in 1926. He observed the differentiation of the epithelium into lens fibers when the anterior one-third of 52-hour-old chick embryo eyes were cultured in vitro.
In 1929 Kirby, Estey, and Tabor found that cultures of chick embryo lens epithelium were more successful in the regular culture media than when the various constituent inorganic salts were increased more than 25% over the normal. They also found an irregular effect of hypertonic media on these cultures, while a 10% to 15% dilution had a marked depressant action on the growth. When they used a slightly more alkaline medium (pH 8.2), they observed a fairly marked depression of the rate of growth in the cultures.
In 1932 Kirby, Estey, and Weiner observed that an increase in the glucose content of the medium produced no real effect on the growth
MAMO JG, LEINFELDER PJ. Growth of Lens Epithelium in CultureI. Characteristics of Growth. AMA Arch Ophthalmol. 1958;59(3):417-419. doi:10.1001/archopht.1958.00940040123014