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Article
May 1959

Staining of the Retina with Saccharated Iron Oxide

Author Affiliations

St. Louis
From the Department of Ophthalmology and the Oscar Johnson Institute, Washington University School of Medicine.

AMA Arch Ophthalmol. 1959;61(5):698-708. doi:10.1001/archopht.1959.00940090700005
Abstract

Introduction  This study deals with retinal flat preparations and histological sections of eyes stained with saccharated iron oxide. In contradistinction to many other routinely employed staining techniques, the method presented here has shown its greatest merits when applied to the nonfixed tissue and, therefore, has produced its best results in vital or supravital specimens.The use of saccharated iron oxide (hereinafter SIO) is facilitated by the availability of commercial preparations for systemic administration of iron.* In principle, the SIO stain is related to Hale's1 colloidal iron stain and its modification by Rinehart and Abdul-Haj.2 The latter technique has recently been applied to the eye by Zimmerman3,4 for demonstration of acid mucopolysaccharides (AMS) in formalinfixed and paraffin-embedded sections. For the purpose of comparison, Zimmerman's approach has been compared in this study with other metachromatic staining techniques, such as Steedman's5 Alcian Blue 8 Gs. With both methods, the

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