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Article
October 1960

Silver Impregnation of Whole Retinas

Author Affiliations

Montreal, Canada
From the Departments of Pathology and Ophthalmology of the Maisonneuve Hospital.

Arch Ophthalmol. 1960;64(4):528-535. doi:10.1001/archopht.1960.01840010530008
Abstract

Although much has been learned about the basic structure of the retina through the use of histoanalytic methods such as silver impregnation (Golgi,1 Cajal2) or methylene blue (Dogiel3), a great deal of work has yet to be done in order to clarify the connections and physiology of its elements. This has been confirmed by recent studies that demonstrate the occurrence of several types of glia (Wolter4) and of exogenous fibers in the retina (Ventura and Mathieu5). Most of the histologic investigations on intraretinal connections have been performed on sectioned specimens, which frequently do not give a clear idea of the extent of these cellular relations. Methylene blue staining of whole-mount retinas has produced good results in the hands of some authors but is unsuitable for studying the fine terminations of cellular processes. This is also true for the Golgi method,6 which, owing to the

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