The recent advances clarifying the role of lipid metabolism in diabetes have produced a resurgence of interest in the study of experimental retinopathy.
Ashton1 states that true diabetic retinopathy has never been produced in the laboratory animal. The experiments of Becker2 utilizing alloxanized rabbits receiving corticotropin (ACTH) could not be duplicated by Marble3 and others.
Unequivocal aneurysmal changes have been demonstrated experimentally in the cat by induced retinal venous thrombosis, not by the metabolic alterations of diabetes alone.4
Microaneurysms may be seen in many disease states,5 and occur randomly in normal preparations of rabbit and human retina. For these reasons a more exact criterion of what constitutes early pathognomonic change in the retinal capillary is a necessity for future animal experimentation.
The literature is replete with methods for flat retina preparation, but only a few techniques have been widely applied.6-13 The popular Hotchkiss-McManus technique
KIRSCHNER R, LEOPOLD IH. Retinal Changes in the Alloxan Diabetic Rat Maintained on a High Fat DietPreliminary Studies Utilizing a New Flat Retina Technique. Arch Ophthalmol. 1960;64(5):681-684. doi:10.1001/archopht.1960.01840010683009