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January 1961

Aqueous Humor Proteins in UveitisImmunoelectrophoretic and Gel Diffusion Studies on Normal and Pathological Human Aqueous Humor

Author Affiliations

New York
From the Department of Surgery (Ophthalmology), The New York Hospital-Cornell Medical Center (Dr. Peretz) and The Rockefeller Institute (Dr. Tomasi).

Arch Ophthalmol. 1961;65(1):20-23. doi:10.1001/archopht.1961.01840020022005

Introduction  The application of immunological techniques such as immunoelectrophoresis and gel diffusion has been of considerable value in determining the distribution and characteristics of proteins in various body fluids.1,2 Serum and urine have been the body fluids most frequently studied.Other workers have noted difficulty in analysis of individual samples of aqueous humor.3,4 This is due to the limited amount of fluid available and the small concentrations of protein present. For these reasons, concentrates or pools of several samples were often made prior to analysis.5,6,7 In the present study the immunological techniques applicable to small volumes of fluid were applied to the analysis of individual specimens. Normal and pathological aqueous humor were both examined and a comparison made between the immunoelectrophoretic pattern of the patient's aqueousMaterials and Methods The 24 patients in this study were seen at The New York Hospital-Cornell Medical Center. Aqueous was obtained

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