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September 1961

Thymidine-Tritium Labeling of the Cellular Elements of the Corneal Stroma

Author Affiliations

Burlington, Vt.

Arch Ophthalmol. 1961;66(3):362-365. doi:10.1001/archopht.1961.00960010364013

When a corneal transplant "takes," a basic problem remains as to which corneal elements are replaced following the graft. The fate of the sulfated mucopolysaccharides of the transplant has been studied by several investigators using S-351,2 but methods are needed to specifically label and locate individual cells in order to study the movement of the cellular elements. In recent years thymidine-tritium has been used in studying the synthesis of desoxyribonucleic acid (DNA). Once incorporated into the nucleus the radioactive label is retained in the DNA which undergoes very slow turnover during the life of the cell. With highresolution autoradiographic film techniques it is possible to study the subsequent history of the labeled nucleus.3 The use of this method is limited because thymidine is taken up only during the premitotic doubling stage. In the case of the corneal epithelium, where there is rapid proliferation, the labeling problem is not

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