Diabetic retinopathy in humans is a major ocular problem. The pathogenesis is unknown and treatment is limited. One approach to this problem is the use of experimental diabetic animals. The present investigation is a study of retinal changes in partially pancreatectomized rats maintained for periods up to 27 months without insulin.
Materials and Method
Albino Wistar type rats were partially pancreatectomized (95%) by Dr. Foglia, University of Buenos Aires, at two to three months of age.1 After selection of affected animals by periodic urine glucose determinations, they were sent to Dr. Lazzarini-Robertson, New York University, for general study. For periods up to 27 months, the animals were maintained without insulin on a 15 to 18 gm daily allotment of Wayne Laboratory Blox diet (24% protein and 4% fat). The animals showed glucose blood levels of 390-480 mg% (Somogyi-Nelson) for more than two hours after the glucose-cortisone tolerance test. The
LEVENE R, LAZZARINI-ROBERTSON A, FOGLIA VG, SINGER J. The Retina in Experimental Diabetic Rats. Arch Ophthalmol. 1963;70(2):253-255. doi:10.1001/archopht.1963.00960050255019