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November 1963

Further Autoradiographic Studies of the Lens EpitheliumNormal and X-Irradiated Rat Eyes

Author Affiliations

Rome, Italy
Former Fellow of the Lederle Foundation (Dr. Scullica).; From the Ophthalmology Branch, National Institute of Neurological Diseases and Blindness, National Institutes of Health, Public Health Service, US Department of Health, Education, and Welfare, Bethesda 14, Md.

Arch Ophthalmol. 1963;70(5):659-665. doi:10.1001/archopht.1963.00960050661015

Introduction  The effect of roentgen irradiation on deoxyribonucleic acid (DNA) synthesis in the epithelium of the rat lens has been investigated recently using autoradiography after intraperitoneal administration of H3-thymidine.1 This study demonstrates that the mitotic inhibition resulting from a dose of 1,000 r x-rays is accompanied by a temporary depression in the number of cells undergoing DNA synthesis. The latter effect appeared to be due mainly to a presynthetic block which prevented new cells from entering the synthesizing (S) period. The results indicated further that the synthetic process is briefly interrupted in some of the cells which were in the S period at the time of irradiation; although, generally, DNA duplication was completed despite irradiation.The aim of the present experiment was to determine the fate of cells irradiated during the synthesizing period. For this purpose, rats of the same strain and age as those previously used

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