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Article
June 1964

Incorporation of Idoxuridine (IDU) by the Rabbit Lens Epithelium in Vitro

Author Affiliations

New York
Departments of ophthalmology and physiology, College of Physicians and Surgeons, Columbia University, and the Brookhaven National Laboratory.

Arch Ophthalmol. 1964;71(6):861-864. doi:10.1001/archopht.1964.00970010877016
Abstract

Previous studies have shown that maintenance of the rabbit lens in a perfusion culture system for two to three days can effect an increase in the number of cells undergoing mitosis and DNA synthesis (thymidine incorporation) in the central area of the lens epithelium.1,2 This "activation" of DNA synthesis and mitosis occurs in an area of the epithelial layer which ordinarily shows very little or no mitotic activity.3 The use of measurements of the incorporation of I131-labeled idoxuridine (5-iodo-2′-deoxyuridine; IDU), an analogue of thymidine,4,14 as an index of cellular proliferation in certain systems has been suggested.5-8,15 The facility of detecting the γ-ray emission of tracer amounts of I131 offers certain advantages, for quantitative measurements, over more commonly used compounds such as C14- and H3-thymidine. Also, there is the possibility of sequential measurements on living tissue. The present paper shows that

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