Various instruments and devices have been designed to hold and fix a rehydrated preserved cornea during the dissection of a lamellar graft. We have been using an effective method of immobilization which requires nothing more than a 20 mm plastic (or glass) sphere and a 3×3 gauze pad, and feel that its simplicity warrants a brief descriptive report.
The preserved cornea is removed from glycerin storage and rehydrated by immersion in saline for five minutes in the standard manner. A 20 mm (enucleation type) plastic sphere is placed on a 3×3 gauze pad. The corners are gathered, the guaze is tightly stretched over the sphere by twisting, and locked in position by binding with multiple loops of a rubber band. The preserved cornea is placed upon the surface and fixed in position with six interrupted 6/0 silk sutures. The lamellar graft is then dissected free following the method outlined
TURTZ AI, BLOCH MH. Lamellar Dissection of Preserved Cornea. Arch Ophthalmol. 1964;72(6):808-810. doi:10.1001/archopht.1964.00970020810013