[Skip to Content]
[Skip to Content Landing]
Article
February 1966

STERILITY AND CRYOSURGERY

Author Affiliations

First Ophthalmological Clinic Budapest, Hungary
National Institute of Public Health Budapest, Hungary

Arch Ophthalmol. 1966;75(2):302-303. doi:10.1001/archopht.1966.00970050304031

This article is only available in the PDF format. Download the PDF to view the article, as well as its associated figures and tables.

Abstract

To the Editor:  We have read with interest Dr. John G. Bellows' letter on sterility and cryosurgery (Arch Ophthal74:290-291, 1965). Our experiences are consistent with his finding that cryogens are practically free of bacteria. We examined samples of liquid nitrogen by evaporating the samples under sterile conditions and subsequent determining germ count in the container. We found one living bacterium for every 5 to 10 ml of liquid nitrogen. It should be taken into account, however, that even the most dangerous bacteria are not at all impaired at the temperature of the liquid nitrogen. We added lyophilized cultures of Pseudomonas aeruginosa and Staphylococcus aureus thoroughly ground with talc to liquid nitrogen and determined the germ count in the container after the nitrogen had evaporated. The bacteria could be recovered in full number in both cases.We have drawn the conclusion that, in spite of the sterility (in the

First Page Preview View Large
First page PDF preview
First page PDF preview
×