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Article
July 1969

THE PERIPHERY OF DESCEMET'S MEMBRANE: A STAINING TECHNIQUE

Author Affiliations

Washington, DC

Arch Ophthalmol. 1969;82(1):144. doi:10.1001/archopht.1969.00990020146031
Abstract

To the Editor.  —The following staining technique has proved useful in differentiating the fiber system at the periphery of Descemet's Membrane described in the Archives (80:197-201 [Aug] 1968).The preparation of the specimen is essentially the same as described in the article referred to above and the staining procedure is carried out on the slide.

Solutions 

Staining Procedure 

  1. Soak with solution 1 for three minutes.

  2. Soak with solution 2 for three minutes.

  3. Soak again with solution 1 for three minutes.

  4. Rinse well with distilled water.

  5. Cover specimen with methylene blue solution for one minute.

  6. Rinse with distilled water.

  7. Cover specimen with fast red S solution.

This last step in the staining process is to be carried out under low-power microscopy so that as soon as the differentiation is clear, the fast red is soaked up and the specimen is mounted in glycerin jelly.

Results  Methylene blue stains the trabecular

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