To the Editor.
—The following staining technique has proved useful in differentiating the fiber system at the periphery of Descemet's Membrane described in the Archives (80:197-201 [Aug] 1968).The preparation of the specimen is essentially the same as described in the article referred to above and the staining procedure is carried out on the slide.
Soak with solution 1 for three minutes.
Soak with solution 2 for three minutes.
Soak again with solution 1 for three minutes.
Rinse well with distilled water.
Cover specimen with methylene blue solution for one minute.
Rinse with distilled water.
Cover specimen with fast red S solution.
Methylene blue stains the trabecular
Preziosi VA. THE PERIPHERY OF DESCEMET'S MEMBRANE: A STAINING TECHNIQUE. Arch Ophthalmol. 1969;82(1):144. doi:10.1001/archopht.1969.00990020146031