[Skip to Content]
[Skip to Content Landing]
September 1969

The Water-Loading Test in RabbitsA Method to Detect Potential Ocular Hypotensive Drugs

Author Affiliations

Fort Worth, Tex
From the sections of toxicology (Mr. McDonald and Borgmann) and pharmacology (Mr. Hodges and Dr. Leaders), Department of Research, Division of Science and Technology, Alcon Laboratories, Inc., Fort Worth, Tex.

Arch Ophthalmol. 1969;82(3):381-384. doi:10.1001/archopht.1969.00990020383017

Tap water (100 ml/kg) was administered by oral gavage to normal adult rabbits. Significant elevation of the intraocular pressure (IOP), lasting for at least 30 minutes, was observed. Following topical treatment, the IOP was significantly reduced by epinephrine bitartrate, carbachol, isoproterenol bitartrate (Isolevin [Britain]), hexamethonium chloride, propranolol hydrochloride, pilocarpine hydrochloride, and physostigmine salicylate, but not by phenoxybenzamine hydrochloride or atropine sulfate. Oral administration of dichlorphenamide or acetazolamide, three hours prior to "water loading," resulted in a lower IOP than that observed for normal control animals. Based on these data, the water-loading test in rabbits appears to represent a valid procedure for screening drugs with potential antiglaucoma activity.