Constant pressure perfusion was done in 18 enucleated human eyes before and after obstruction of Schlemm's canal. Obstruction was accomplished by the retrograde injection of siliconerubber compound via the aqueous veins. Decrease in outflow facility followed fractional obstruction of Schlemm's canal with linear correlation between percentage circumference filled and percentage decrease in C value. Outflow was through aqueous veins draining the unobstructed segment of the canal. Complete circumferential filling of the canal did not result in 100% reduction of aqueous outflow facility. The average decrease was 83%. Fluorescein perfusion showed escape routes via the scleral emissaria of the anterior ciliary arteries and vortex veins (one eye) and the sclera paralimbally (all eyes), equatorially and posteriorly (two eyes). Dissection revealed fluorescent fluid in the suprachoroid with staining of ciliary body, anterior vitreous and overlying sclera.
Jocson VL, Sears ML. Experimental Aqueous Perfusion in Enucleated Human EyesResults After Obstruction of Schlemm's Canal. Arch Ophthalmol. 1971;86(1):65-71. doi:10.1001/archopht.1971.01000010067013