Anterior segment necrosis was induced in rabbits by extensive cautery of both long posterior ciliary arteries. Metabolic and histologic changes were studied at intervals after operation. Severe depression of glucose levels in corneal stroma and aqueous lasted for two days after cautery. Lactate levels, initially very high, fell below normal at one week but had returned to normal at six weeks. Stromal hydration was elevated for one week after cautery, then became normal. Corneal epithelial glycogen was severely depleted one and two days after operation, but epithelium otherwise appeared normal. Ischemia of anterior segment was reversed after three to six weeks but irreversible changes of iris, ciliary body, and lens persisted. It is concluded that corneal complications seen in anterior segment necrosis stem from changes in aqueous metabolic components resulting from severely reduced aqueous turnover.
Pfister RR, Friend J, Dohlman CH. Anterior Segment Necrosis in RabbitsMetabolic and Histologic Changes. Arch Ophthalmol. 1971;86(3):301-307. doi:10.1001/archopht.1971.01000010303013