Fluorescein arrival in each ocular fundus subsequent to intravenous injection was plotted simultaneously in humans by a fluorophotometric method. In 17 normal subjects arrival time was equal in the two eyes; the resultant dye dilution curves were symmetric in shape when simultaneous curves of right and left eyes were compared. In eight patients with proven unilateral carotid artery occlusion and ten others with probable unilateral carotid artery or ocular circulatory disease, there was either delay in arrival of dye to the affected eye or asymmetry of the resultant dye dilution curves. Potential diagnostic and investigative uses of this apparatus in the study of circulation are suggested.
Winkelman JZ, Zappia RJ, Gay AJ. Human Arm to Retina Circulation TimeDetermination by Simultaneous Fluorophotometry. Arch Ophthalmol. 1971;86(6):626-636. doi:10.1001/archopht.1971.01000010628004