Metabolic data obtained in the present study indicate that the technique of Kaufman and Capella for cryopreservation results in a metabolic insult to corneal tissue. The overall energy-yielding metabolism of the cryopreserved tissue is reduced 13% compared with that of fresh tissue, with a shift toward a more glycolytic-dependent metabolism in the cryopreserved cornea. The glycolytic to respiratory glucose catabolism ratio for fresh tissue is 88:12, while the ratio for the cryopreserved tissue is 96:4. This metabolic insult results from freeze- or thaw-induced injury, or both, and is not due to either the cryoprotective solutions or to mechanical trauma to the tissue by its manipulation during the procedure. Further, the metabolic insult of cryopreservation appears to be minimal when compared to the insult produced by quick-freezing, a process that results in an 88% decrease in overall metabolism with the tissue becoming 100% anaerobic. The same metabolic alterations appear to occur in both the human and rabbit cornea.
Geroski DH, Edelhauser HF. Metabolic Evaluation of Cryopreserved Corneal Tissue. Arch Ophthalmol. 1974;91(2):130-133. doi:10.1001/archopht.1974.03900060136011