A tissue culture method for the mass production of rabbit corneal endothelium includes early trypsinization of explant cultures. With this technique, sufficient corneal endothelial cells may be obtained for biochemical investigation.
Perlman M, Baum JL. The Mass Culture of Rabbit Corneal Endothelial Cells. Arch Ophthalmol. 1974;92(3):235-237. doi:10.1001/archopht.1974.01010010243014