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December 1974

The Ultrastructure of Human Organ-Cultured CorneaI. Endothelium

Author Affiliations

From the Department of Ophthalmology, University of Minnesota, Minneapolis (Drs. Doughman, Harris, Miller, and Lindstrom), the Research Service, Veteran's Administration Center, Wood, Wis (Dr. Van Horn), the departments of ophthalmology and physiology, Medical College of Wisconsin, Milwaukee (Dr. Van Horn), and the Memorial Sloan-Kettering Cancer Center, New York (Dr. Good).

Arch Ophthalmol. 1974;92(6):516-523. doi:10.1001/archopht.1974.01010010530015

Human corneal endothelial cells maintained ultrastructural integrity after organ culture storage at 37 C for 10 to 21 days. Those organ-cultured corneas with postmortem times less than 12 hours had a complete layer of ultrastructurally intact endothelial cells of normal thickness and diameter. When postmortem times were longer than 24 hours, there was a complete layer of ultrastructurally intact endothelial cells that were large in diameter and thin. Control fellow corneas in moist chamber storage at 4 C demonstrated endothelial cell disruption proportional in area and severity to postmortem time duration. This implies that a dynamic process of endothelial repair, presumably by enlargement of remaining viable cells, occurs in organ culture.