A light and electron microscopical study of subretinal fluid removed at surgery for rhegmatogenous retinal detachment showed a heterogeneity of cell types originating from the neural retina, pigment epithelium, and wandering cells of the blood. Subcellular organelles sedimenting in the centrifuged pellets could be identified as to cell of origin, health of the parent cell, and time since cell rupture. Acellular specimens were typical of angiomatous detachments or long-standing rhegmatogenous detachments. Because various mechanisms of cellular pathophysiology contribute to the destructive and reparative processes during retinal detachment, the cytologic analysis of subretinal fluid becomes a useful tool in understanding the biological processes that affect visual recovery or impairment after successful retinal detachment surgery.
Feeney L, Burns RP, Mixon RM. Human Subretinal FluidIts Cellular and Subcellular Components. Arch Ophthalmol. 1975;93(1):62-69. doi:10.1001/archopht.1975.01010020066012