• Some aqueous humor constituents, including ascorbate, protein, and sodium, potassium, and calcium ions, were analyzed in three groups of adult buphthalmic (bu/bu) rabbits from strain AXBU/J and in control rabbits of the closely related strain AX/J.
Aqueous humor ascorbate concentration of the normal strain was 22.5 ± 2.3 mg/100 ml. The buphthalmic rabbits that had no meaningful clinical signs of glaucoma at the time of sample collection had lower values (11.7 ± 1.7 mg/100 ml). Much lower values were observed in the bu/bu mutant rabbits that showed mild to severe glaucoma: 8.9 ± 3.8 mg/100 ml and 1.9 ± 0.4 mg/100 ml, respectively. It was concluded that reduction of aqueous ascorbate is a biochemical change associated with the pathological development of buphthalmia in rabbits of the AXBU/J strain. However, the exact mechanism is still unclear.
Lam K, Lee P, Fox R. Aqueous Ascorbate Concentration in Hereditary Buphthalmic Rabbits. Arch Ophthalmol. 1976;94(9):1565-1567. doi:10.1001/archopht.1976.03910040395015