• In a method for the routine examination of the endothelium of donor corneas before transplantation, the donor endothelial cells are examined and photographed as they undergo preservation or culture in liquid media. A specular microscope with a modified objective lens is used (working distance, approximately 9 mm). The donor corneas are kept in a special storage container that allows a direct view of the endothelium from outside the container. The endothelium of any cornea preserved in a liquid medium may thus be examined and photographed at high magnification without opening the storage container.
Bourne WM. Examination and Photography of Donor Corneal Endothelium. Arch Ophthalmol. 1976;94(10):1799-1800. doi:10.1001/archopht.1976.03910040573017