[Skip to Content]
[Skip to Content Landing]
January 1978

An Enzyme Histochemical Analysis of Human Subretinal Fluid

Author Affiliations

From the Departments of Ophthalmology (Dr Feman) and Biochemistry (Dr Lam), Albany Medical College of Union University, Albany, NY.

Arch Ophthalmol. 1978;96(1):129-131. doi:10.1001/archopht.1978.03910050085022

• During rhegmatogenous retinal detachment, two major phagocytic cell populations can be identified in the subretinal fluid. Routine light and electron microscopic studies may be inadequate to assess quantitatively the free-floating retinal pigment epithelial cells and the macrophagic retinal pigment-laden cells. Enzyme histochemical staining permits a simple, rapid, reproducible method of identifying each cell type. This cytologic biochemical differentiation is an important tool for reexamination of the theories associated with subretinal fluid cell activity.