To the Editor.
—In the article, "An Improved Method for Practice Vitrectomy" (Archives 96:521-525,1978), the authors reported that the intravitreal injection of 0.02 mL of 10% fluorescein sodium, which quickly diffuses through the rabbit vitreous and stains it yellowish green, is one method of improving visualization of clear vitreous during experimental vitrectomy. Electroretinogram (ERG) findings produced by our laboratory suggest that while intravitreal fluorescein may be helpful in aiding the novice vitreous surgeon in learning good technique, it should not be used for vitreous staining when vitrectomy is being performed for scientific instead of practice purposes.In our experiment, two adult female Dutch belted rabbits were initially sedated with ketamine hydrochloride, 75 mg/kg, and resedated every 45 minutes with 25 mg/kg of ketamine hydrochloride. The pupils were dilated with 1% tropicamide and 10% phenylephrine hydrochloride. After dark adaptation for 20 minutes, silver wire electrodes were placed on the
Moorhead LC. Practice Vitrectomy. Arch Ophthalmol. 1980;98(7):1297-1298. doi:10.1001/archopht.1980.01020040149027