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February 1981

Quantitative Determination of T Cells in Ocular Lymphoid InfiltratesAn Indirect Method for Distinguishing Between Pseudolymphomas and Malignant Lymphomas

Author Affiliations

From the Department of Pathology, Columbia University, College of Physicians and Surgeons, New York (Dr Knowles); the Departments of Pathology and Ophthalmology, Cornell University Medical College, and the Department of Pathology, Manhattan Eye, Ear and Throat Hospital, New York (Dr Jakobiec).

Arch Ophthalmol. 1981;99(2):309-316. doi:10.1001/archopht.1981.03930010311020

• T lymphocytes may be identified by two comparatively simple techniques: sheep erythrocyte (E) rosette formation and cytochemically demonstrable acid α-naphthyl acetate esterase (ANAE) activity. We tested the quantitative determination of T cells in 17 ocular lymphoid tumors as an indirect method for characterizing their clonality. Six lesions containing greater than 40% T cells (47% to 73%; mean, 62%) were shown to be polyclonal proliferations and were classified as pseudolymphomas by histologic criteria. Seven lesions containing less than 30% T cells (3% to 20%; mean, 10%) were shown to be monoclonal B-cell proliferations and were classified as malignant lymphomas by histologic criteria. Only two lesions containing between 30% and 40% T cells could not be unequivocally assigned to the monoclonal or polyclonal category solely based on the percentage of T cells. In the final two lesions, the tissue specimen was too small to allow a full panel of immunologic studies; both tumors showed a predominance of T cells consistent with their benign histologic features.