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March 1982

Fluorescein Binding to Normal Human Serum Proteins Demonstrated by Equilibrium Dialysis

Author Affiliations

From the Scheie Eye Institute, Department of Ophthalmology, University of Pennsylvania Medical School, Philadelphia.

Arch Ophthalmol. 1982;100(3):484-487. doi:10.1001/archopht.1982.01030030486024

• The binding of fluorescein to normal human serum proteins in a physiologic solvent at 37 °C was measured by equilibrium dialysis. Human serum contained 3.28 x 10-3M concentration fluoresceinbinding sites, with an average association constant at 37 °C of 0.54 X 104M-1. The percentage of total fluorescein bound by human serum proteins ranged from 83% to 53% when the total fluorescein concentration ranged from 6.9 X 10-5 to 6.2 X 10-3M.