This article is only available in the PDF format. Download the PDF to view the article, as well as its associated figures and tables.
To the Editor.
—The article by Drs Gibralter and Jakobiec entitled "Supravital and Vital Staining of Diseased Corneal Endothelium in Whole-Mount Preparations," in the August Archives (1982;100:1308-1312), described a new staining technique (a combination of trypan blue and alizarin red S) for quantitative evaluation of corneal endothelial cells. The method was first described by us in Investigative Ophthalmology (1976;15:1000-1002).
Spence D, Peyman GA. Diseased Corneal Endothelium. Arch Ophthalmol. 1983;101(3):488. doi:10.1001/archopht.1983.01040010488044