—We regret that our computerized and manual literature searches did not turn up the article by Drs Spence and Peyman that indeed provided the first description of combined staining with trypan blue and alizarin red S for delineating normal and abnormal corneal endothelium. In their zeal to put the historical record straight, they do us a disservice by implying that we touted this staining method as new and as having been invented by ourselves whereas, in fact, the fourth reference in our introduction credits the antecedent work of Sperling1 on this particular combination of stains. In all likelihood, Sperling, and Spence and Peyman, independently evaluated the utility of these stains, and in close temporal proximity, reported their findings without knowledge of each other's work. Our article was simply an attempt to amplify the value of these stains as applied to a wide spectrum of corneal endothelial conditions.
Jakobiec FA, Gibralter RP. Diseased Corneal Endothelium-Reply. Arch Ophthalmol. 1983;101(3):488. doi:10.1001/archopht.1983.01040010488045