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—I can understand Drs Weaver and Isenberg's concern for experimental design. However, our study was not intended to generate data regarding comparable dose regimens for each drug, but was actually constructed to provide information of aqueous levels following repeated frequent applications. This method was chosen to provide the best opportunity of detecting any level of antiviral levels, given the well-known previous studies on poor penetration into human aqueous in corneas with intact epithelium. The recommended dosages, of course, are determined for patients with herpetic keratitis and have no relevance for this type of study.Regarding vehicles and penetration, the pitfalls of comparing aqueous levels in rabbits to those in humans are well known. Acyclovir is not soluble enough for eyedrop formulation, and, since it was given as an ointment, the irrigation fluid was assayed to separate any likelihood of spurious levels obtained from residual drug in the cul-de-sac.
Poirier RH. Intraocular Antiviral Penetration-Reply. Arch Ophthalmol. 1983;101(7):1146. doi:10.1001/archopht.1983.01040020148032