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Article
February 1984

McCarey-Kaufman (MK) Organ Culture and MK Medium-Shifted Corneas

Author Affiliations

From the Department of Ophthalmology, University of Minnesota, Minneapolis (Drs Nelson, Lange, Lindstrom, and Doughman); St Paul-Ramsey Medical Center (Dr Nelson); the Research Service, Veterans Administration Medical Center, Wood, Wis (Dr Hatchell); and the Departments of Ophthalmology and Physiology, Medical College of Wisconsin, Milwaukee (Dr Hatchell).; Dr Hatchell is a Research to Prevent Blindness, Inc, and William and Mary Greve International Research scholar.

Arch Ophthalmol. 1984;102(2):308-311. doi:10.1001/archopht.1984.01040030252036
Abstract

• Paired human donor corneas were studied to determine the effects of temperature, osmolarity, and media shifts on the endothelium of corneas stored in McCarey-Kaufman (MK) medium initially and then shifted to standard organ culture medium. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) demonstrated intact corneal endothelium in six of seven human control corneas stored in MK medium prior to fixation. Intact corneal endothelium was similarly demonstrated by specular microscopy, SEM, and TEM in seven of seven "MK-shifted" corneas. These data suggest that MK-stored donor corneas may be transferred into an organ culture system at 34 °C for prolonged storage with maintenance of corneal endothelial cell viability.

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