[Skip to Content]
[Skip to Content Landing]
Article
November 1984

Energy Dispersive X-ray Analysis of the CorneaApplication to Paraffin Sections of Normal and Diseased Corneas

Author Affiliations

From the Departments of Ophthalmology (Drs Robinson and Streeten) and Pathology (Dr Streeten), State University of New York Upstate Medical Center, Syracuse.

Arch Ophthalmol. 1984;102(11):1678-1682. doi:10.1001/archopht.1984.01040031362028
Abstract

• The distribution of chemical elements in the normal human cornea was studied by energy dispersive x-ray analysis and scanning electron microscopy of routinely prepared paraffin sections. Calcium, phosphorus, and sulfur were consistently present in quantities above background and varied in concentration regionally. Analysis of fresh-frozen tissue, an approximation of the in vivo state, gave a similar elemental profile to paraffin sections, except for the loss of diffusable electrolytes in the latter. After fixation, S was the most abundant element and was highest in Descemet's membrane. Corneas with granular, lattice, macular, and Fuchs' endothelial dystrophies, band keratopathy, and spheroidal degeneration were also examined. Characteristic patterns of abnormal S and Ca distribution were found in each of the dystrophies. The relative proportions of Ca, P, and S gave diagnostic profiles for distinguishing band keratopathy and spheroidal degeneration.

×