[Skip to Content]
Access to paid content on this site is currently suspended due to excessive activity being detected from your IP address 54.205.173.230. Please contact the publisher to request reinstatement.
[Skip to Content Landing]
Article
May 1985

A Rat Model of Bacterial KeratitisEffect of Antibiotics and Corticosteroid

Author Affiliations

From the Departments of Ophthalmology (Mr Badenoch and Drs Hay and Coster) and Clinical Microbiology (Dr McDonald), Flinders Medical Centre, Bedford Park, South Australia.

Arch Ophthalmol. 1985;103(5):718-722. doi:10.1001/archopht.1985.01050050110028
Abstract

• A model of bacterial keratitis in rats was developed to quantify the effect of antibiotics and corticosteroid on the infective process. Corneas were inoculated with Staphylococcus aureus, Pseudomonas aeruginosa, or Streptococcus pneumoniae. The natural history of infection with these organisms was determined. Groups of animals received topical antibiotics and prednisolone acetate. The effect of treatment on the number of leukocytes and viable bacteria in the corneas was determined. Prednisolone did not influence the effect of the antibiotics; however, steroid treatment alone increased the pseudomonad count as much as 20-fold above the count in untreated eyes. In general, both the antibiotic and steroid treatments were more successful when begun eight hours after infection than when begun at 24 hours. A 1% gentamicin sulfate preparation proved effective against each of the infections, including a pneumococcal strain considered resistant on the basis of in vitro tests.

×