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—Dr Refojo points out that presence of undenatured proteins on the lenses does not preclude the presence of denatured proteins. We agree with him and would like to stress that we did not make statements to the contrary in our aforereferenced article, "Identification of Proteins in Contact Lens Surface Deposits by Immunofluorescence Microscopy."Dr Refojo's second comment was regarding the staining of proteins in the material or matrix of the lens. We would like to explain that the lenses are cut transversely. The cut side (not only the outer surface) is also treated with the antisera. Therefore, there would be a good possibility of detectable staining if there were any undenatured proteins inside the material.As stated in our article, the relationship of cleaning and disinfection of the lenses to deposits was outside the scope of the study. However, we agree that processes of disinfection, especially heat disinfection,
Allansmith MR. Identification of Proteins in Contact Lens Surface Deposits by Immunofluorescence Microscopy-Reply. Arch Ophthalmol. 1985;103(9):1270. doi:10.1001/archopht.1985.01050090019004